Full metadata record
DC Field | Value | Language |
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dc.contributor.author | Lee, Hyunkyoung | - |
dc.contributor.author | Baek, Jiyeon | - |
dc.contributor.author | Min, Hyunjung | - |
dc.contributor.author | Cho, Ik-Hyun | - |
dc.contributor.author | Yu, Seong-Woo | - |
dc.contributor.author | Lee, Sung Joong | - |
dc.date.available | 2017-07-05T08:47:23Z | - |
dc.date.created | 2017-04-10 | - |
dc.date.issued | 2017-01 | - |
dc.identifier.issn | 1021-7401 | - |
dc.identifier.uri | http://hdl.handle.net/20.500.11750/2309 | - |
dc.description.abstract | Objective: It is well known that Schwann cells play an important role in Wallerian degeneration after peripheral nerve injury. Previously, we reported that toll-like receptor 3 (TLR3) is expressed on Schwann cells, implicating its role in Schwann cell activation during Wallerian degeneration. In this study, we tested this possibility using TLR3 knock-out mice. Methods: Sciatic nerve-crush injury was induced in wild-type and TLR3 knock-out mice. Histological sections of the sciatic nerve were analyzed for Wallerian degeneration on days 3 and 7 after injury. The level of macrophage infiltration was measured by real-time RT-PCR, flow cytometry and immunohistochemistry. The macrophage-recruiting chemokine gene expressions in the injured nerve were determined by real-time RT-PCR. Results: In TLR3 knock-out mice, the nerve injury-induced axonal degeneration and subsequent axonal debris clearance were reduced compared to in wild-type mice. In addition, nerve injury-induced macrophage infiltration into injury sites was attenuated in TLR3 knock-out mice and was accompanied by reduced expression of macrophage-recruiting chemokines such as CC-chemokine ligands (CCL)2/MCP-1, CCL4/MIP-1β and CCL5/RANTES. These macrophage-recruiting chemokines were induced in primary Schwann cells upon TLR3 stimulation. Finally, intraneural injection of polyinosinic-polycytidylic acid, a synthetic TLR3 agonist, induced macrophage infiltration into the sciatic nerve in vivo. Conclusion: These data show that TLR3 signaling contributes to Wallerian degeneration after peripheral nerve injury by affecting Schwann cell activation and macrophage recruitment to injured nerves. © 2016 S. Karger AG, Basel. | - |
dc.language | English | - |
dc.publisher | Karger | - |
dc.title | Toll-Like Receptor 3 Contributes to Wallerian Degeneration after Peripheral Nerve Injury | - |
dc.type | Article | - |
dc.identifier.doi | 10.1159/000449134 | - |
dc.identifier.scopusid | 2-s2.0-84992735200 | - |
dc.identifier.bibliographicCitation | NeuroImmunoModulation, v.23, no.4, pp.209 - 216 | - |
dc.description.isOpenAccess | FALSE | - |
dc.subject.keywordAuthor | Toll-like receptor 3 | - |
dc.subject.keywordAuthor | Schwann cells | - |
dc.subject.keywordAuthor | Wallerian degeneration | - |
dc.subject.keywordAuthor | Sciatic nerve injury | - |
dc.subject.keywordAuthor | Chemokine | - |
dc.subject.keywordAuthor | Macrophage recruitment | - |
dc.subject.keywordPlus | DOUBLE-STRANDED-RNA | - |
dc.subject.keywordPlus | SCHWANN-CELLS | - |
dc.subject.keywordPlus | INFLAMMATORY EVENTS | - |
dc.subject.keywordPlus | GROWTH-FACTOR | - |
dc.subject.keywordPlus | REGENERATION | - |
dc.subject.keywordPlus | DEMYELINATION | - |
dc.subject.keywordPlus | ACTIVATION | - |
dc.subject.keywordPlus | SYSTEM | - |
dc.subject.keywordPlus | REMYELINATION | - |
dc.subject.keywordPlus | MACROPHAGES | - |
dc.citation.endPage | 216 | - |
dc.citation.number | 4 | - |
dc.citation.startPage | 209 | - |
dc.citation.title | NeuroImmunoModulation | - |
dc.citation.volume | 23 | - |
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