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dc.contributor.author Min, SR[Min, Sung Ran] ko
dc.contributor.author Davarpanah, SJ[Davarpanah, Seyed Javad] ko
dc.contributor.author Jung, SH[Jung, Seo Hee] ko
dc.contributor.author Park, YI[Park, Youn-il] ko
dc.contributor.author Liu, JR[Liu, Jang R.] ko
dc.contributor.author Jeong, WJ[Jeong, Won-Joong] ko
dc.date.available 2017-07-11T04:41:39Z -
dc.date.created 2017-04-10 -
dc.date.issued 2015-11 -
dc.identifier.citation Plant Biotechnology Reports, v.9, no.6, pp.443 - 449 -
dc.identifier.issn 1863-5466 -
dc.identifier.uri http://hdl.handle.net/20.500.11750/2584 -
dc.description.abstract We developed a new plastid transformation vector system using the putative replication origin of a minicircular chromosome from the marine dinoflagellate Heterocapsatriquetra. Transplastomic tobacco plants generated with this vector properly expressed the green fluorescent protein (GFP) gene without incorporating it into the plastid genome. To construct the episomal vector, a 610-bp DNA fragment containing the putative replication origin was fused to a dicistronic expression cassette encoding the aminoglycoside 3′-adenyltransferase (aadA) and gfp genes under control of the plastid rrn promoter. The vector was delivered to plastids of tobacco leaf explants by biolistic bombardment. After 8 weeks of bombardment, episomal transformant shoots were generated from leaf explants cultured on selection media containing 500 mg/L spectinomycin. Fluorescence microscopy and northern blot analysis demonstrated GFP expression in episomal transformant plants. PCR, Southern blot analysis, recovery of episomes, and sequencing analysis showed the vector to be maintained as self-replicating extrachromosomal circular DNA molecules for at least 6 months. Using a single construct for all plants, our episomal vector system may offer an advantage over the conventional plastid vector systems, which require species-specific constructs. © 2015, Korean Society for Plant Biotechnology and Springer Japan. -
dc.publisher SPRINGER -
dc.subject Episome -
dc.subject Heterocapsatriquetra -
dc.subject Plastid Transformation -
dc.subject Tobacco -
dc.title An episomal vector system for plastid transformation in higher plants -
dc.type Article -
dc.identifier.doi 10.1007/s11816-015-0381-4 -
dc.identifier.wosid 000367069000010 -
dc.identifier.scopusid 2-s2.0-84948568684 -
dc.type.local Article(Overseas) -
dc.type.rims ART -
dc.description.journalClass 1 -
dc.contributor.nonIdAuthor Min, SR[Min, Sung Ran] -
dc.contributor.nonIdAuthor Davarpanah, SJ[Davarpanah, Seyed Javad] -
dc.contributor.nonIdAuthor Jung, SH[Jung, Seo Hee] -
dc.contributor.nonIdAuthor Park, YI[Park, Youn-il] -
dc.contributor.nonIdAuthor Jeong, WJ[Jeong, Won-Joong] -
dc.identifier.citationVolume 9 -
dc.identifier.citationNumber 6 -
dc.identifier.citationStartPage 443 -
dc.identifier.citationEndPage 449 -
dc.identifier.citationTitle Plant Biotechnology Reports -
dc.type.journalArticle Article -
dc.contributor.affiliatedAuthor Liu, JR[Liu, Jang R.] -
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