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Amperometric hydrogen peroxide and cholesterol biosensors designed by using hierarchical curtailed silver flowers functionalized graphene and enzymes deposits

Amperometric hydrogen peroxide and cholesterol biosensors designed by using hierarchical curtailed silver flowers functionalized graphene and enzymes deposits
Nalini, S[Nalini, Seetharamaiah]Nandini, S[Nandini, Seetharamaiah]Shanmugam, S[Shanmugam, Sangaraju]Neelagund, SE[Neelagund, Shivayogeeswar E.]Melo, JS[Melo, Jose Savio]Suresh, GS[Suresh, Gurukar Shivappa]
DGIST Authors
Shanmugam, S[Shanmugam, Sangaraju]
Issue Date
Journal of Solid State Electrochemistry, 18(3), 685-701
Article Type
Activation EnergyBiosensorsCholesterolCholesterol OxidaseChronoamperometryCyclic VoltammetryDifferential Pulse VoltammetryEnzyme ImmobilizationFunctionalized GrapheneGalvanostatic ElectrodepositionGalvanostaticsGrapheneGraphite ElectrodesHorse-Radish PeroxidaseHydrogen PeroxidePeroxidesScanning Electron MicroscopySilverSilver Deposits
Novel flower-like silver particles with triangular plates as building block along with functionalized graphene (straggled sheets) and enzymes horseradish peroxidase (HRP) or cholesterol oxidase (ChOx), were obtained on graphite electrode by galvanostatic electrodeposition method. The morphology of the electrodeposits has been characterized using scanning electron microscopy and energy-dispersive analysis of X-ray. The resulting biosensors named Nf/(HRP-f-graphene-Ag)/Gr and Nf/(ChOx-f-graphene-Ag)/Gr were evaluated for electrochemical activity using cyclic voltammetry (CV), differential pulse voltammetry (DPV) and chronoamperometry. Optimization of the interdependent experimental parameters such as pH and temperature were achieved and maintained constant throughout the experiments. An activation energy of 2.5 kJ mol -1 was obtained for Nf/(HRP-f-graphene-Ag)/Gr electrode while Nf/(ChOx-f-graphene-Ag)/Gr showed an activation energy of 2.06 and 3.12 kJ mol-1. Furthermore, the former electrode demonstrated a good linear range of 25 μM to 19.35 mM with rapid response time of 3 s and detection limit of 5 μM for hydrogen peroxide. Similarly, the Nf/(ChOx-f-graphene-Ag)/ Gr electrode revealed a linear range of 0.1-4.5 mM with rapid response time of 3 s and an excellent detection limit of 0.514 mM for cholesterol. Besides this, the Nf/(HRP-f-graphene-Ag)/Gr and Nf/(ChOx-f-graphene-Ag)/Gr electrodes displayed a Michaelis-Menten constant of 0.26 and 0.57 mM, respectively, suggesting high affinity and enzymatic activity. The enhanced performance of biosensors towards detection of substrate and rejection of interferents, provided an evidence for its high anti-interference ability. Additionally the biosensors exhibit long term storage stability and reproducibility with antifouling properties. © 2013 Springer-Verlag Berlin Heidelberg.
Related Researcher
  • Author Shanmugam, Sangaraju Advanced Energy Materials Laboratory
  • Research Interests Electrocatalysts for fuel cells; water splitting; metal-air batteries; Polymer electrolyte membranes for fuel cells; flow batteries; Hydrogen generation and utilization
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Department of Energy Science and EngineeringAdvanced Energy Materials Laboratory1. Journal Articles

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