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AMPK ACTIVATION REGULATES NEURONAL STRUCTURE IN DEVELOPING HIPPOCAMPAL NEURONS
- AMPK ACTIVATION REGULATES NEURONAL STRUCTURE IN DEVELOPING HIPPOCAMPAL NEURONS
- Ramamurthy, S[Ramamurthy, S.]; Chang, E[Chang, E.]; Cao, Y[Cao, Y.]; Zhu, J[Zhu, J.]; Ronnett, GV[Ronnett, G. V.]
- DGIST Authors
- Ronnett, GV[Ronnett, G. V.]
- Issue Date
- Neuroscience, 259, 13-24
- Article Type
- 4′,6-Diamidino-2-Phenylindole; 5-Aminoimidazole-4-Carboxamide-1-B-D-Ribofuranoside; 5′-Adenosine Monophosphate-Activated Protein Kinase; Adenosine Diphosphate; Adenosine Monophosphate; Adenosine Triphosphate; ADP; Age Factors; AICAR; Aminoimidazole Carboxamide; AMP; AMP-Activated Protein Kinases; AMPK; Animal Cell; Animals; Animals, Newborn; ATP; Axon; Bovine Serum Albumin; Brain-Specific Kinase; Brain Nerve Cell; BRSK; BSA; Calcium/Calmodulin-Dependent Kinase Kinase Beta; CaMKK Beta; Cell Energy; Cell Stress; Cells, Cultured; Controlled Study; DAPI; Days In Vitro; Dendrite; Dendrites; Development; DIV; DPBS; Dulbecco' s Phosphate-Buffered Saline; Embryo, Mammalian; Enzyme Activation; Enzyme Activity; Enzyme Regulation; Enzyme Structure; Enzyme Subunit; Female; Gene Expression Regulation, Developmental; Glycogen Synthase Kinase-3Beta; GSK-3 Beta; Hank' s Balanced Salt Solution; HBSS; Hippocampal Neurons; Hippocampus; HN; Hydroxymethylglutaryl Coenzyme A Reductase Kinase; Hydroxymethylglutaryl Coenzyme A Reductase Kinase Alpha1; Hydroxymethylglutaryl Coenzyme A Reductase Kinase Alpha2; Hydroxymethylglutaryl Coenzyme A Reductase Kinase Beta1; Hydroxymethylglutaryl Coenzyme A Reductase Kinase Beta2; Hydroxymethylglutaryl Coenzyme A Reductase Kinase Gamma1; Hydroxymethylglutaryl Coenzyme A Reductase Kinase Gamma2A; Hydroxymethylglutaryl Coenzyme A Reductase Kinase Gamma2B; Hypoglycemic Agents; Knockout; KO; Liver Kinase B1; LKB1; Male; Mammalian Target of Rapamycin; Map2; Metabolism; Mice; Mice, Inbred C57BL; Mice, Transgenic; Microtubule-Associated Protein 2; MTOR; Nerve Cell Differentiation; Nerve Fiber Growth; Neurites; Neuroanatomy; Neuron; Neurons; Non-Human; P13K; P70 Ribosomal Protein S6 Kinase; P70S6K; Phosphatidyl Inositol 3-Kinase; Polyvinylidene Difluoride; Priority Journal; Protein Expression; Protein Kinase B; PVDF; Rat; Reverse Transcriptase-Polymerase Chain Reaction; RT-PCR; SDS; Signal Transduction; Sodium Dodecyl Sulfate; TBST; Tris-Buffered Saline+Tween 20; Unclassified Drug; Wild Type; WT
- AMP-activated protein kinase (AMPK) is a serine/threonine kinase that functions as a cellular and whole organism energy sensor to regulate ATP-consuming (anabolic) and ATP-generating (catabolic) pathways. The heterotrimeric AMPK complex consists of a catalytic α-subunit, regulatory β-subunit, and an AMP/ATP-binding γ-subunit. Several alternate isoforms exist for each subunit (α1, α2, β1, β2, γ1, γ2 and γ3). However, little is known of the expression pattern or function of the individual catalytic complexes in regulating neuronal structure. In this study, we examined the role of AMPK subunits in differentiating hippocampal neurons. We found that during development, the expression of AMPK subunits increase and that activation of AMPK by energetic stress inhibits neuronal development at multiple stages, not only during axon outgrowth, but also during dendrite growth and arborization. The presence of a single functional AMPK catalytic complex was sufficient to mediate these inhibitory effects of energetic stress. Activation of AMPK mediates these effects by suppressing both the mTOR and Akt signaling pathways. These findings demonstrate that the energy-sensing AMPK pathway regulates neuronal structure in distinct regions of developing neurons at multiple stages of development. © 2013 IBRO.
- Elsevier Ltd
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