Full metadata record
DC Field | Value | Language |
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dc.contributor.author | Oh, Yong-Seok | - |
dc.contributor.author | Bae, Sun Sik | - |
dc.contributor.author | Park, Jong Bae | - |
dc.contributor.author | Ha, Sang Hoon | - |
dc.contributor.author | Ryu, Sung Ho | - |
dc.contributor.author | Suh, Pann-Ghill | - |
dc.date.available | 2017-07-11T05:42:34Z | - |
dc.date.created | 2017-04-10 | - |
dc.date.issued | 2015-12 | - |
dc.identifier.issn | 1932-6203 | - |
dc.identifier.uri | http://hdl.handle.net/20.500.11750/2800 | - |
dc.description.abstract | Sphingosine kinase is a lipid kinase that converts sphingosine into sphingosine-1-phosphate, an important signaling molecule with intracellular and extracellular functions. Although diverse extracellular stimuli influence cellular sphingosine kinase activity, the molecular mechanisms underlying its regulation remain to be clarified. In this study, we investigated the phosphorylation-dependent regulation of mouse sphingosine kinase (mSK) isoforms 1 and 2. mSK1a was robustly phosphorylated in response to extracellular stimuli such as phorbol ester, whereas mSK2 exhibited a high basal level of phosphorylation in quiescent cells regardless of agonist stimulation. Interestingly, phorbol ester-induced phosphorylation of mSK1a correlated with suppression of its activity. Chemical inhibition of conventional PKCs (cPKCs) abolished mSK1a phosphorylation, while overexpression of PKCα, a cPKC isoform, potentiated the phosphorylation, in response to phorbol ester. Furthermore, an in vitro kinase assay showed that PKCα directly phosphorylated mSK1a. In addition, phosphopeptide mapping analysis determined that the S373 residue of mSK1a was the only site phosphorylated by cPKC. Interestingly, alanine substitution of S373 made mSK1a refractory to the inhibitory effect of phorbol esters, whereas glutamate substitution of the same residue resulted in a significant reduction in mSK1a activity, suggesting the significant role of this phosphorylation event. Taken together, we propose that mSK1a is negatively regulated through cPKC-dependent phosphorylation at S373 residue. © 2015 Oh et al. This is an open access article distributed under the terms of the Creative Commons Attribution License, which permits unrestricted use, distribution, and reproduction in any medium, provided the original author and source are credited. | - |
dc.language | English | - |
dc.publisher | Public Library of Science | - |
dc.title | Mouse Sphingosine Kinase 1a Is Negatively Regulated through Conventional PKC-Dependent Phosphorylation at S373 Residue | - |
dc.type | Article | - |
dc.identifier.doi | 10.1371/journal.pone.0143695 | - |
dc.identifier.scopusid | 2-s2.0-84955499678 | - |
dc.identifier.bibliographicCitation | PLoS ONE, v.10, no.12 | - |
dc.description.isOpenAccess | TRUE | - |
dc.subject.keywordPlus | 1-PHOSPHATE | - |
dc.subject.keywordPlus | Alanine | - |
dc.subject.keywordPlus | Amino ACID Substitution | - |
dc.subject.keywordPlus | Article | - |
dc.subject.keywordPlus | CALCIUM MOBILIZATION | - |
dc.subject.keywordPlus | CELLULAR-REGULATION | - |
dc.subject.keywordPlus | Controlled Study | - |
dc.subject.keywordPlus | DISTINCT ROLES | - |
dc.subject.keywordPlus | Enzyme Activation | - |
dc.subject.keywordPlus | Enzyme Activity | - |
dc.subject.keywordPlus | Enzyme Assay | - |
dc.subject.keywordPlus | Enzyme Inhibition | - |
dc.subject.keywordPlus | Enzyme Phosphorylation | - |
dc.subject.keywordPlus | Enzyme Regulation | - |
dc.subject.keywordPlus | Extracellular Matrix | - |
dc.subject.keywordPlus | FACTOR-INDUCED ACTIVATION | - |
dc.subject.keywordPlus | FUNCTIONAL-CHARACTERIZATION | - |
dc.subject.keywordPlus | Glutamic ACID | - |
dc.subject.keywordPlus | In Vitro Study | - |
dc.subject.keywordPlus | MOLECULAR-CLONING | - |
dc.subject.keywordPlus | Mouse | - |
dc.subject.keywordPlus | Nonhuman | - |
dc.subject.keywordPlus | PATHWAY | - |
dc.subject.keywordPlus | Phorbol Ester | - |
dc.subject.keywordPlus | Phosphopeptide | - |
dc.subject.keywordPlus | Protein Expression | - |
dc.subject.keywordPlus | Protein Kinase C | - |
dc.subject.keywordPlus | Protein Kinase C Alpha | - |
dc.subject.keywordPlus | Protein Protein Interaction | - |
dc.subject.keywordPlus | RECEPTOR | - |
dc.subject.keywordPlus | Residue Analysis | - |
dc.subject.keywordPlus | Serine | - |
dc.subject.keywordPlus | SPHINGOSINE-1-PHOSPHATE | - |
dc.subject.keywordPlus | Sphingosine Kinase 1 | - |
dc.subject.keywordPlus | Sphingosine Kinase 1A | - |
dc.subject.keywordPlus | Sphingosine Kinase 2 | - |
dc.subject.keywordPlus | Unclassified Drug | - |
dc.citation.number | 12 | - |
dc.citation.title | PLoS ONE | - |
dc.citation.volume | 10 | - |
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