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dc.contributor.author Lee, Hye Hyeon -
dc.contributor.author Han, Min Ho -
dc.contributor.author Hwang, Hye Jin -
dc.contributor.author Kim, Gi-Young -
dc.contributor.author Moon, Sung-Kwon -
dc.contributor.author Hyun, Jin-Won -
dc.contributor.author Kim, Wun-Jae -
dc.contributor.author Choi, Yung Hyun -
dc.date.available 2017-07-11T06:00:43Z -
dc.date.created 2017-04-10 -
dc.date.issued 2015-02 -
dc.identifier.issn 1107-3756 -
dc.identifier.uri http://hdl.handle.net/20.500.11750/2937 -
dc.description.abstract Diallyl trisulfide (DATS; di-2-propen-1-yl trisulfide) is an organic polysulfide compound found in garlic and other allium vegetables. Although certain studies have demonstrated that DATS possesses strong anti-inflammatory activity, the underlying molecular mechanisms remain largely unresolved. In the present study, the anti-inflammatory potential of DATS was investigated using the murine macrophage RAW 264.7 cell model. At non-toxic concentrations, DATS inhibited the production of nitric oxide (NO) and prostaglandin E2 by inhibiting inducible NO synthase and cyclooxygenase-2 expression at the transcriptional level in lipopolysaccharide (LPS)-activated RAW 264.7 macrophages. DATS attenuated the release of the pro-inflammatory cytokines, tumor necrosis factor-α and interleukin-1β, by inhibiting mRNA expression, respectively. DATS also suppressed LPS-induced DNA-binding activity of nuclear factor-κB (NF-κB), as well as the nuclear translocation of the NF-κB p65, which correlated with the inhibitory effects of DATS on inhibitor-κB (IκB) degradation. In addition, DATS was observed to significantly suppress LPS-induced Toll-like receptor 4 (TLR4) and myeloid differentiation factor 88 expression and the binding of LPS to macrophages, indicating the antagonistic effect of DATS against TLR4. Furthermore, blocking TLR4 signaling with the specific TLR4 signaling inhibitor, CLI-095, increased the anti-inflammatory potential of DATS in LPS-stimulated RAW 264.7 macrophages. These data demonstrate that DATS may attenuate the initiation of LPS-mediated intracellular signaling cascades by suppressing activation of NF-κB and by inhibiting binding of LPS to TLR4 on macrophages. © International Journal of Molecular Medicine 2014. -
dc.publisher Spandidos Publications -
dc.title Diallyl trisulfide exerts anti-inflammatory effects in lipopolysaccharide-stimulated RAW 264.7 macrophages by suppressing the Toll-like receptor 4/nuclear factor-kappa B pathway -
dc.type Article -
dc.identifier.doi 10.3892/ijmm.2014.2036 -
dc.identifier.scopusid 2-s2.0-84919680292 -
dc.identifier.bibliographicCitation International Journal of Molecular Medicine, v.35, no.2, pp.487 - 495 -
dc.subject.keywordAuthor diallyl trisulfide -
dc.subject.keywordAuthor inflammation -
dc.subject.keywordAuthor nuclear factor-kappa B -
dc.subject.keywordAuthor Toll-like receptor 4 -
dc.subject.keywordPlus GARLIC ORGANOSULFUR COMPOUNDS -
dc.subject.keywordPlus RAW-264.7 MACROPHAGES -
dc.subject.keywordPlus MOLECULAR-MECHANISMS -
dc.subject.keywordPlus SIGNAL-TRANSDUCTION -
dc.subject.keywordPlus INFLAMMATION -
dc.subject.keywordPlus EXPRESSION -
dc.subject.keywordPlus TLR4 -
dc.subject.keywordPlus ACTIVATION -
dc.subject.keywordPlus INDUCTION -
dc.subject.keywordPlus CANCER -
dc.citation.endPage 495 -
dc.citation.number 2 -
dc.citation.startPage 487 -
dc.citation.title International Journal of Molecular Medicine -
dc.citation.volume 35 -
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Department of New Biology ETC 1. Journal Articles
ETC 1. Journal Articles

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