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Planar Hall magnetoresistive aptasensor for thrombin detection

Title
Planar Hall magnetoresistive aptasensor for thrombin detection
Author(s)
Sinha, B[Sinha, B.]Ramulu, TS[Ramulu, T. S.]Kim, KW[Kim, K. W.]Venu, R[Venu, R.]Lee, JJ[Lee, J. J.]Kim, CG[Kim, C. G.]
DGIST Authors
Ramulu, TS[Ramulu, T. S.]Kim, KW[Kim, K. W.]Venu, R[Venu, R.]Kim, CG[Kim, C. G.]
Issued Date
2014-09-15
Type
Article
Article Type
Article
Subject
AptamerAptamers, NucleotideAptasensorBiosensing TechniquesBiosensorChemistryClinical DiagnosisClinical DiagnosticsColorimetryConcentration (Parameters)DevicesDiagnosisDimeticoneDNAElectric PotentialElectrochemistryEnzyme AssayEnzymesEquipment DesignEvaluation StudyFluorescenceGenetic ProceduresGold DepositsHigh Signal-to-Noise RatioHumanHumansLimit of DetectionLower Detection LimitMagnetic Nano-ParticleMagnetite NanoparticleMagnetite NanoparticlesMagneto Resistive SensorsMorphologyPlanar Hall Magnetoresistive AptasensorPlanar Hall Magnetoresistive SensorProtein AnalysisProteinsQuartz Crystal MicrobalanceSandwichScanning Electron MicroscopySensorsSignal-to-Noise RatioSignal Noise RatioSiliconesStreptavidinSuperparamagneticsSurface Plasmon ResonanceThrombinThrombin Aptamer
ISSN
0956-5663
Abstract
The use of aptamer-based assays is an emerging and attractive approach in disease research and clinical diagnostics. A sensitive aptamer-based sandwich-type sensor is presented to detect human thrombin using a planar Hall magnetoresistive (PHR) sensor in cooperation with superparamagnetic labels. A PHR sensor has the great advantages of a high signal-to-noise ratio, a small offset voltage and linear response in the low-field region, allowing it to act as a high-resolution biosensor. In the system presented here, the sensor has an active area of 50. μm×50. μm with a 10-nm gold layer deposited onto the sensor surface prior to the binding of thiolated DNA primary aptamer. A polydimethylsiloxane well of 600-μm radius and 1-mm height was prepared around the sensor surface to maintain the same specific area and volume for each sensor. The sensor response was traced in real time upon the addition of streptavidin-functionalized magnetic labels on the sensor. A linear response to the thrombin concentration in the range of 86. pM-8.6. μM and a lower detection limit down to 86. pM was achieved by the proposed present method with a sample volume consumption of 2. μl. The proposed aptasensor has a strong potential for application in clinical diagnosis. © 2014 Elsevier B.V.
URI
http://hdl.handle.net/20.500.11750/3035
DOI
10.1016/j.bios.2014.03.021
Publisher
Elsevier Ltd
Related Researcher
  • 김철기 Kim, CheolGi 화학물리학과
  • Research Interests Magnetic Materials and Spintronics; Converging Technology of Nanomaterials and Biomaterials; Bio-NEMS;MEMS
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Appears in Collections:
Department of Physics and Chemistry Lab for NanoBio-Materials & SpinTronics(nBEST) 1. Journal Articles
Department of Physics and Chemistry ETC 1. Journal Articles

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