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Identification of Novel Autoantibodies in Type 1 Diabetic Patients Using a High-Density Protein Microarray
- Identification of Novel Autoantibodies in Type 1 Diabetic Patients Using a High-Density Protein Microarray
- Koo, BK[Koo, Bo Kyung]; Chae, S[Chae, Sehyun]; Kim, KM[Kim, Kristine M.]; Kang, MJ[Kang, Min Jueng]; Kim, EG[Kim, Eunhee G.]; Kwak, SH[Kwak, Soo Heon]; Jung, HS[Jung, Hye Seung]; Cho, YM[Cho, Young Min]; Choi, SH[Choi, Sung Hee]; Park, YJ[Park, Young Joo]; Shin, CH[Shin, Choong Ho]; Jang, HC[Jang, Hak C.]; Shin, CS[Shin, Chan Soo]; Hwang, D[Hwang, Daehee]; Yi, EC[Yi, Eugene C.]; Park, KS[Park, Kyong Soo]
- DGIST Authors
- Hwang, D[Hwang, Daehee]
- Issue Date
- Diabetes, 63(9), 3022-3032
- Article Type
- Adult; Aged; Antibody Titer; Autoantibodies; Autoantibody; Blood Sampling; Child; Child, Preschool; Controlled Study; Diabetes Mellitus, Type 1; Diabetes Mellitus, Type 2; eef1a1 Autoantibody; Enzyme Linked Immunosorbent Assay; Female; Glucose Tolerance; Glutamate Decarboxylase; Graves Disease; Human; Humans; Immunoprecipitation; Insulin Dependent Diabetes Mellitus; Major Clinical Study; Male; Middle Aged; Non-Insulin Dependent Diabetes Mellitus; Peptide Elongation Factor 1; Prevalence; Priority Journal; Protein Analysis; Protein Array Analysis; Protein Microarray; UBE2L3 Autoantibody; Ubiquitin-Conjugating Enzymes; Unclassified Drug
- Autoantibodies can facilitate diagnostic and therapeutic means for type 1 diabetes (T1DM). We profiled autoantibodies from serum samples of 16 T1DM patients, 16 type 2 diabetic (T2DM) patients, and 27 healthy control subjects with normal glucose tolerance (NGT) by using protein microarrays containing 9,480 proteins. Two novel autoantibodies, anti-EEF1A1 and anti-UBE2L3, were selected from microarrays followed by immunofluorescence staining of pancreas. We then tested the validity of the candidates by ELISA in two independent test cohorts: 1) 95 adults with T1DM, 49 with T2DM, 11 with latent autoimmune diabetes in adults (LADA), 20 with Graves disease, and 66 with NGT and 2) 33 children with T1DM and 34 healthy children. Concentrations of these autoantibodies were significantly higher in T1DM patients than in NGT and T2DM subjects (P < 0.01), which was also confirmed in the test cohort of children (P < 0.05). Prevalence of anti-EEF1A1 and anti-UBE2L3 antibodies was 29.5% and 35.8% in T1DM, respectively. Of note, 40.9% of T1DM patients who lack anti-GAD antibodies (GADA) had anti-EEF1A1 and/or anti-UBE2L3 antibodies. These were also detected in patients with fulminant T1DM but not LADA. Our approach identified autoantibodies that can provide a new dimension of information indicative of T1DM independent of GADA and new insights into diagnosis and classification of T1DM. © 2014 by the American Diabetes Association.
- American Diabetes Association Inc.
- Related Researcher
Systems Biology and Medicine Lab
Multilayered spatiotemporal networks; Regulatory motifs or pathways; Metabolite-protein networks; Network stochasticity; Proteomics and informatics
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- Department of New BiologySystems Biology and Medicine Lab1. Journal Articles
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