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Morphogenetic and neuronal characterization of human neuroblastoma multicellular spheroids cultured under undifferentiated and all-trans-retinoic acid-differentiated conditions
- Morphogenetic and neuronal characterization of human neuroblastoma multicellular spheroids cultured under undifferentiated and all-trans-retinoic acid-differentiated conditions
- Jung, Gwon-Soo; Lee, Kyeong-Min; Park, Jin-Kyu; Choi, Seong-Kyoon; Jeon, Won Bae
- DGIST Authors
- Lee, Kyeong-Min; Jeon, Won Bae
- Issue Date
- BMB Reports, 46(5), 276-281
- Article Type
- Antineoplastic Agent; Antineoplastic Agents; Blotting, Western; Cell Adhesion Molecules; Cell Culture; Cell Differentiation; Cell Proliferation; Cell Survival; Cells, Cultured; Drug Effect; Extracellular Matrix; Human; Humans; Multicellular Neurospheroids; Multicellular Spheroid; Neurite; Neurites; Neuroblastoma; Neuroblastoma Cells; Neuronal Differentiation; Polymerase Chain Reaction; Retinoic Acid; Spheroids, Cellular; Tretinoin; Ultrastructure; Western Blotting
- In this study, we aimed to compare the morphogenetic and neuronal characteristics between monolayer cells and spheroids. For this purpose, we established spheroid formation by growing SH-SY5Y cells on the hydrophobic surfaces of thermally-collapsed elastin-like polypeptide. After 4 days of culture, the relative proliferation of the cells within spheroids was approximately 92% of the values for monolayer cultures. As measured by quantitative assays for mRNA and protein expressions, the production of synaptophysin and neuronspecific enolase (NSE) as well as the contents of cell adhesion molecules (CAMs) and extracellular matrix (ECM) proteins are much higher in spheroids than in monolayer cells. Under the all-trans-retinoic acid (RA)-induced differentiation condition, spheroids extended neurites and further up-regulated the expression of synaptophysin, NSE, CAMs, and ECM proteins. Our data indicate that RA-differentiated SH-SY5Y neurospheroids are functionally matured neuronal architectures. © 2013 by the The Korean Society for Biochemistry and Molecular Biology.
- Korean Society for Biochemistry and Molecular Biology
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- Companion Diagnostics and Medical Technology Research Group1. Journal Articles
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