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dc.contributor.author Jung, Gwon-Soo -
dc.contributor.author Lee, Kyeong-Min -
dc.contributor.author Park, Jin-Kyu -
dc.contributor.author Choi, Seong-Kyoon -
dc.contributor.author Jeon, Won Bae -
dc.date.available 2017-07-11T06:35:57Z -
dc.date.created 2017-04-10 -
dc.date.created 2017-04-10 -
dc.date.created 2017-04-10 -
dc.date.created 2017-04-10 -
dc.date.created 2017-04-10 -
dc.date.created 2017-04-10 -
dc.date.issued 2013-05 -
dc.identifier.citation BMB Reports, v.46, no.5, pp.276 - 281 -
dc.identifier.issn 1976-6696 -
dc.identifier.uri http://hdl.handle.net/20.500.11750/3235 -
dc.description.abstract In this study, we aimed to compare the morphogenetic and neuronal characteristics between monolayer cells and spheroids. For this purpose, we established spheroid formation by growing SH-SY5Y cells on the hydrophobic surfaces of thermally-collapsed elastin-like polypeptide. After 4 days of culture, the relative proliferation of the cells within spheroids was approximately 92% of the values for monolayer cultures. As measured by quantitative assays for mRNA and protein expressions, the production of synaptophysin and neuronspecific enolase (NSE) as well as the contents of cell adhesion molecules (CAMs) and extracellular matrix (ECM) proteins are much higher in spheroids than in monolayer cells. Under the all-trans-retinoic acid (RA)-induced differentiation condition, spheroids extended neurites and further up-regulated the expression of synaptophysin, NSE, CAMs, and ECM proteins. Our data indicate that RA-differentiated SH-SY5Y neurospheroids are functionally matured neuronal architectures. © 2013 by the The Korean Society for Biochemistry and Molecular Biology. -
dc.language English -
dc.publisher Korean Society for Biochemistry and Molecular Biology -
dc.title Morphogenetic and neuronal characterization of human neuroblastoma multicellular spheroids cultured under undifferentiated and all-trans-retinoic acid-differentiated conditions -
dc.type Article -
dc.identifier.doi 10.5483/BMBRep.2013.46.5.196 -
dc.identifier.wosid 000324919300007 -
dc.identifier.scopusid 2-s2.0-84880629496 -
dc.type.local Article(Overseas) -
dc.type.rims ART -
dc.description.journalClass 1 -
dc.citation.publicationname BMB Reports -
dc.identifier.kciid ART001770934 -
dc.contributor.localauthor Jung, Gwon-Soo -
dc.contributor.localauthor Lee, Kyeong-Min -
dc.contributor.localauthor Park, Jin-Kyu -
dc.contributor.localauthor Choi, Seong-Kyoon -
dc.contributor.localauthor Jeon, Won Bae -
dc.contributor.nonIdAuthor Jung, Gwon-Soo -
dc.contributor.nonIdAuthor Park, Jin-Kyu -
dc.identifier.citationVolume 46 -
dc.identifier.citationNumber 5 -
dc.identifier.citationStartPage 276 -
dc.identifier.citationEndPage 281 -
dc.identifier.citationTitle BMB Reports -
dc.type.journalArticle Article -
dc.description.isOpenAccess Y -
dc.subject.keywordAuthor Cell adhesion molecules -
dc.subject.keywordAuthor Extracellular matrix -
dc.subject.keywordAuthor Multicellular neurospheroids -
dc.subject.keywordAuthor Neuroblastoma cells -
dc.subject.keywordAuthor Neuronal differentiation -
dc.subject.keywordPlus EXTRACELLULAR-MATRIX -
dc.subject.keywordPlus CELL-ADHESION -
dc.subject.keywordPlus NEURITE OUTGROWTH -
dc.subject.keywordPlus UP-REGULATION -
dc.subject.keywordPlus N-CADHERIN -
dc.subject.keywordPlus MODEL -
dc.subject.keywordPlus APOPTOSIS -
dc.subject.keywordPlus NCAM -

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