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Sensitivity Enhancement of a Miniaturized Surface Plasmon Resonance Sensor Using Combination of Self-Assembled Mono layer and Protein G
- Sensitivity Enhancement of a Miniaturized Surface Plasmon Resonance Sensor Using Combination of Self-Assembled Mono layer and Protein G
- Sin, EJ[Sin, Eun Jung]; Lim, JO[Lim, Jeong-Ok]; Sohn, YS[Sohn, Young-Soo]
- DGIST Authors
- Sin, EJ[Sin, Eun Jung]
- Issue Date
- Nanoscience and Nanotechnology Letters, 4(8), 821-826
- Article Type
- Antibodies; Bovine Serum Albumins; C-Reactive Proteins; Cardiovascular Event; Chemical Detection; Chemical Treatments; CRP; Detection Capability; Early Diagnosis; Flow Channels; Immunosensor; Immunosensors; Linker Molecules; Protein G; SAM; SAM Layer; Self Assembled Monolayers; Sensitivity Enhancements; Sensors; SPR; SPR Sensor Chip; SPR Sensors; SPR Systems; Surface Plasmon Resonance; Surface Plasmon Resonance Sensor
- The detection capabilities of surface plasmon resonance (SPR) sensor chips modified with protein G, a self-assembled monolayer (SAM), and a combination of protein G and a SAM were investigated and compared using a miniaturized SPR sensor system. The miniaturized SPR system had two flow channels, with one used as a reference. To immobilize the antibodies on the SPR sensor chip for the detection of C-reactive protein (CRP), different linker molecules were formed on the gold SPR sensor chip at the beginning of the experiment. The CRP has been reported as a biomarker of future cardiovascular events. The antibody of CRP and bovine serum albumin (BSA) were then sequentially injected. The responses to CRP at concentrations of 0.5, 1, 3, and 5 μg/ml were observed and the sensitivities of each system compared. The SPR sensor chips modified with the protein G on the SAM layer exhibited the highest sensitivity and a stable linearity. The results demonstrated the feasibility of using the proposed SPR immunosensors for detecting CRP for early diagnosis without any additional chemical treatments. Copyright © 2012 American Scientific Publishers.
- American Scientific Publishers
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