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DC Field Value Language Jeong, Hoe-Su - Bhin, Jinhyuk - Kim, Hyung Joon - Hwang, Daehee - Lee, Dong Ryul - Kim, Kye-Seong - 2017-08-10T08:15:20Z - 2017-08-09 - 2017-04 -
dc.identifier.citation Experimental and Molecular Medicine, v.49 -
dc.identifier.issn 1226-3613 -
dc.identifier.uri -
dc.description.abstract Spermatogonial stem cells (SSCs) are germline stem cells located along the basement membrane of seminiferous tubules in testes. Recently, SSCs were shown to be reprogrammed into multipotent SSCs (mSSCs). However, both the key factors and biological networks underlying this reprogramming remain elusive. Here, we present transcriptional regulatory networks (TRNs) that control cellular processes related to the SSC-to-mSSC reprogramming. Previously, we established intermediate SSCs (iSSCs) undergoing the transition to mSSCs and generated gene expression profiles of SSCs, iSSCs and mSSCs. By comparing these profiles, we identified 2643 genes that were up-regulated during the reprogramming process and 15 key transcription factors (TFs) that regulate these genes. Using the TF-target relationships, we developed TRNs describing how these TFs regulate three pluripotency-related processes (cell proliferation, stem cell maintenance and epigenetic regulation) during the reprogramming. The TRNs showed that 4 of the 15 TFs (Oct4/Pou5f1, Cux1, Zfp143 and E2f4) regulated cell proliferation during the early stages of reprogramming, whereas 11 TFs (Oct4/Pou5f1, Foxm1, Cux1, Zfp143, Trp53, E2f4, Esrrb, Nfyb, Nanog, Sox2 and Klf4) regulated the three pluripotency-related processes during the late stages of reprogramming. Our TRNs provide a model for the temporally coordinated transcriptional regulation of pluripotency-related processes during the SSC-to-mSSC reprogramming, which can be further tested in detailed functional studies. -
dc.publisher 생화학분자생물학회 -
dc.title Transcriptional regulatory networks underlying the reprogramming of spermatogonial stem cells to multipotent stem cells -
dc.type Article -
dc.identifier.doi 10.1038/emm.2017.2 -
dc.identifier.wosid 000404157000004 -
dc.identifier.scopusid 2-s2.0-85033710766 -
dc.type.local Article(Overseas) -
dc.type.rims ART -
dc.description.journalClass 1 -
dc.citation.publicationname Experimental and Molecular Medicine -
dc.identifier.kciid ART002218709 -
dc.contributor.nonIdAuthor Jeong, Hoe-Su -
dc.contributor.nonIdAuthor Bhin, Jinhyuk -
dc.contributor.nonIdAuthor Kim, Hyung Joon -
dc.contributor.nonIdAuthor Lee, Dong Ryul -
dc.contributor.nonIdAuthor Kim, Kye-Seong -
dc.identifier.citationVolume 49 -
dc.identifier.citationTitle Experimental and Molecular Medicine -
dc.type.journalArticle Article -
dc.description.isOpenAccess N -
dc.subject.keywordPlus TRANSFER-RNA SYNTHETASES -
dc.subject.keywordPlus PLURIPOTENCY -
dc.subject.keywordPlus TUMORIGENICITY -
dc.subject.keywordPlus GENERATION -
dc.subject.keywordPlus BIOLOGY -
dc.contributor.affiliatedAuthor Jeong, Hoe-Su -
dc.contributor.affiliatedAuthor Bhin, Jinhyuk -
dc.contributor.affiliatedAuthor Kim, Hyung Joon -
dc.contributor.affiliatedAuthor Hwang, Daehee -
dc.contributor.affiliatedAuthor Lee, Dong Ryul -
dc.contributor.affiliatedAuthor Kim, Kye-Seong -
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Department of New Biology Systems Biology and Medicine Lab 1. Journal Articles


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