Full metadata record
DC Field | Value | Language |
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dc.contributor.author | Kim, Hyunmin | - |
dc.contributor.author | Kim, Do-Young | - |
dc.contributor.author | Joo, Kyung-Il | - |
dc.contributor.author | Kim, Jung-Hye | - |
dc.contributor.author | Jeong, Soon Moon | - |
dc.contributor.author | Lee, Eun Seong | - |
dc.contributor.author | Hahm, Jeong-Hoon | - |
dc.contributor.author | Kim, Kyuhyung | - |
dc.contributor.author | Moon, Dae Woon | - |
dc.date.available | 2017-09-11T04:39:56Z | - |
dc.date.created | 2017-09-11 | - |
dc.date.issued | 2017-08 | - |
dc.identifier.issn | 2045-2322 | - |
dc.identifier.uri | http://hdl.handle.net/20.500.11750/4438 | - |
dc.description.abstract | In this study, we used spectrally focused coherent anti-Stokes Raman scattering (spCARS) microscopy assisted by sum-frequency generation (SFG) to monitor the variations in the structural morphology and molecular vibrations of a live muscle of Caenorhabditis elegans. The subunits of the muscle sarcomeres, such as the M-line, myosin, dense body, and α-actinin, were alternatively observed using spCARS microscopy for different sample orientations, with the guidance of a myosin positional marker captured by SFG microscopy. Interestingly enough, the beam polarization dependence of the spCARS contrasts for two parallel subunits (dense body and myosin) showed a ~90° phase difference. The chemically sensitive spCARS spectra induced by the time-varying overlap of two pulses allowed (after a robust subtraction of the non-resonant background using a modified Kramers-Krönig transformation method) high-fidelity detection of various genetically modified muscle sarcomeres tuned to the C-H vibration (2800-3100 cm-1). Conversely, SFG image mapping assisted by phase-retrieved spCARS spectra also facilitated label-free monitoring of the changes in the muscle content of C. elegans that are associated with aging, based on the hypothesis that the C-H vibrational modes could serve as qualitative chemical markers sensitive to the amount and/or structural modulation of the muscle. © 2017 The Author(s). | - |
dc.language | English | - |
dc.publisher | Nature Publishing Group | - |
dc.title | Coherent raman imaging of live muscle sarcomeres assisted by SFG microscopy | - |
dc.type | Article | - |
dc.identifier.doi | 10.1038/s41598-017-09571-w | - |
dc.identifier.wosid | 000408428400012 | - |
dc.identifier.scopusid | 2-s2.0-85027966341 | - |
dc.identifier.bibliographicCitation | Scientific Reports, v.7, no.1 | - |
dc.description.isOpenAccess | FALSE | - |
dc.subject.keywordPlus | 2ND-HARMONIC GENERATION | - |
dc.subject.keywordPlus | CAENORHABDITIS-ELEGANS | - |
dc.subject.keywordPlus | SCATTERING | - |
dc.subject.keywordPlus | SPECTROSCOPY | - |
dc.subject.keywordPlus | PROTEINS | - |
dc.subject.keywordPlus | COLLAGEN | - |
dc.subject.keywordPlus | BINDING | - |
dc.subject.keywordPlus | MODEL | - |
dc.subject.keywordPlus | BAND | - |
dc.citation.number | 1 | - |
dc.citation.title | Scientific Reports | - |
dc.citation.volume | 7 | - |
dc.description.journalRegisteredClass | scie | - |
dc.description.journalRegisteredClass | scopus | - |
dc.relation.journalResearchArea | Science & Technology - Other Topics | - |
dc.relation.journalWebOfScienceCategory | Multidisciplinary Sciences | - |
dc.type.docType | Article | - |
10.1038_s41598_017_09571_w.pdf
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