On-Demand Selection of Bio-Conjugated Microparticles by Multifarious Micromagnet System
- Title
- On-Demand Selection of Bio-Conjugated Microparticles by Multifarious Micromagnet System
- Author(s)
- Hyeonseol Kim
- DGIST Authors
- Hyeonseol Kim ; CheolGi Kim ; Chun-Yeol You
- Advisor
- 김철기
- Co-Advisor(s)
- Chun-Yeol You
- Issued Date
- 2023
- Awarded Date
- 2023-02-01
- Type
- Thesis
- Description
- Magnetophoresis, Selective particle transport, Label-free manipulation, Biomolecule detection, Micromagnet thin film
- Table Of Contents
-
I. Introduction 1
1.1 Development of microfabrication technology and the Bio-MEMS 1
1.2 Necessity and type of single cell manipulation technologies 2
1.2.1 Electric method 2
1.2.2 Optical method 5
1.2.3 Acoustic method 9
1.2.4 Magnetic method 12
1.3 Cell manipulation technology supported by micromagnet structures 14
1.4 Thesis objectives and outline 18
II. Experimental methods 19
2.1 Fabrication of the micromagnet pattern 19
2.2 Fabrication of nanoscale topography on the micromagnet 21
2.2.1 Fabrication of the non-magnetic topographic structures 21
2.2.2 Surface gradient measurement using interference rings and FE-SEM 21
2.3 Preparation of magnetized cells and micro objects as cell replacement 22
2.3.1 Materials information for the experiments 22
2.3.2 Magnetized polystyrene particles by the magnetic labeling 22
2.3.3 Cell culture and magnetized single cell by the magnetic labeling 22
2.4 Experimental set-up 23
III. Theoretical background 26
3.1 Characteristics of superparamagnetic Particles 26
3.2 Types and fundamentals of magnetophoresis 27
3.2.1 Positive magnetophoresis 29
3.2.2 Negative magnetophoresis 31
3.3 Fundamentals of magnetophoretic circuits 32
3.4 Characteristics of magnetophoretic circuit elements 35
3.4.1 Particle around the disk micromagnet pattern 35
3.4.2 Particle around the conductor-like micromagnet pattern 38
3.4.3 Particle around the diode-like micromagnet pattern 40
3.4.4 Particle around the transistor-like micromagnet pattern 42
3.4.5 Various other magnetophoretic circuits 44
3.5 Analysis using magnetic simulations 45
3.5.1 Micromagnetic simulation, Mumax3 45
3.5.2 Equation-based MATLAB code 46
3.6 Limits of existing magnetophoretic circuits 48
3.7 New concepts to overcome limitations of magnetophoretic circuits 52
3.7.1 Local magnetic energy modification by non-magnetic topography 53
3.7.2 Transport elements independent of the external field conditions 53
3.7.3 Label-free manipulation of the single cell and individual storage 53
3.7.4 Overcoming limited usage of magnetophoretic circuits 54
IV. Result and discussion 55
4.1 Orbital-like particle motion via topographic effects 55
4.1.1 Current modes by matter orbitals and topographic effects of micro hills 58
4.1.2 Orbital departure via forced phase adjustment and the corresponding devices 62
4.1.3 Integrated device with mode switching and return of the particle to the orbital 66
4.1.4 Current-like colloidal current behaviors by magnetic and topographic effects 74
4.2 Area-dependent directionality enhancement of the magnetic particles 79
4.2.1 Universal driving fields of the microrobot and its corresponding directionality 80
4.2.2 Directional control through the combination of unit structures 83
4.2.3 Unit texture and magnetic field optimization that controls magnetic directionality 86
4.2.4 Multifarious trajectory movement at the complex structure 88
4.2.5 Integrated particle collector with a partially different directionality 91
4.2.6 Closed loop by using selective trapping and releasing particles 96
4.3 Label-free manipulation by Negative magnetophoretic circuits 98
4.3.1 Working principle of PsD hole manipulation 99
4.3.2 Circuitry elements for PsD hole transportation 100
4.3.3 Switching efficiency of PsD holes at eclipse diode with anti-dot 103
4.3.4 High-throughput storage capacitors of PsD holes 105
4.4 Motion-based biomarker detection by micromagnet array 112
4.4.1 Biomarker detection principle using disk micromagnets and magnetic particles 113
4.4.2 Tethering trajectories due to the molecular chain and particle size difference 115
4.4.3 Rotating trajectories due to the micromagnet and optimization of variable factors 116
V. Conclusion 120
5.1 Summary of my works 120
5.2 Conclusion of my works 123
References 124
요약문 130
- URI
-
http://hdl.handle.net/20.500.11750/45679
http://dgist.dcollection.net/common/orgView/200000656122
- Degree
- Doctor
- Department
- Department of Physics and Chemistry
- Publisher
- DGIST
- Related Researcher
-
-
Kim, CheolGi
- Research Interests Magnetic Materials and Spintronics; Converging Technology of Nanomaterials and Biomaterials; Bio-NEMS;MEMS
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- Appears in Collections:
- Department of Physics and Chemistry Theses Ph.D.
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