Development of monoclonal antibodies for the functional modulation of human TREM2 signaling
- Title
- Development of monoclonal antibodies for the functional modulation of human TREM2 signaling
- Alternative Title
- 인간 TREM2 신호전달의 기능적 조절을 위한 단클론 항체의 개발
- Author(s)
- Eunbi Kim
- DGIST Authors
- Eunbi Kim ; Kyuhyung Kim ; Hyun-Ho Lim
- Advisor
- 김규형
- Co-Advisor(s)
- Hyun-Ho Lim
- Issued Date
- 2023
- Awarded Date
- 2023-02-01
- Type
- Thesis
- Description
- TREM2, Alzheimer’s disease, microglia, therapeutic, monoclonal antibodies, hybridoma
- Table Of Contents
-
Ⅰ. INTRODUCTION 1
Ⅱ. MATERIALS AND METHODS 5
2.1 Expression and Purification of hTREM2174 5
2.2 Proteolytic digestion of hTREM2174 6
2.3 Immunization and hybridoma fusion 6
2.4 ELISA (Enzyme-linked immunosorbent assay) 7
2.5 Purification of TREM2 monoclonal antibody 8
2.6 Sequence determination of TREM2 monoclonal antibodies 8
2.7 Mouse genotyping 10
Ⅲ. RESULTS 12
3.1 Purification of TREM2 ectodomain 12
3.2 Antibody generation against hTREM2 ectodomain 17
3.3 Antibody subtyping and production 21
3.4 Genotyping of the hTREM2 knock-in mouse 27
Ⅳ. DISCUSSION 30
Ⅴ. REFERENCES 33
Ⅵ. Abstract in Korea (요약문) 35
- URI
-
http://hdl.handle.net/20.500.11750/45701
http://dgist.dcollection.net/common/orgView/200000658331
- Degree
- Master
- Department
- Department of Brain Sciences
- Publisher
- DGIST
- Related Researcher
-
-
Kim, Kyuhyung
- Research Interests Neurobehavior; Neural Circuit; Neurodevelopment; 신경회로; 신경행동; 신경발생; 신경유전학
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- Files in This Item:
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- Appears in Collections:
- Department of Brain Sciences Theses Master
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