Title

Development of monoclonal antibodies for the functional modulation of human TREM2 signaling

Alternative Title

인간 TREM2 신호전달의 기능적 조절을 위한 단클론 항체의 개발

DGIST Authors

Eunbi Kim ; Kyuhyung Kim ; Hyun-Ho Lim

Advisor

김규형

Co-Advisor(s)

Hyun-Ho Lim

Issued Date

2023

Awarded Date

2023-02-01

Citation

Eunbi Kim. (2023). Development of monoclonal antibodies for the functional modulation of human TREM2 signaling. doi: 10.22677/THESIS.200000658331

Type

Thesis

Description

TREM2, Alzheimer’s disease, microglia, therapeutic, monoclonal antibodies, hybridoma

Table Of Contents

Ⅰ. INTRODUCTION 1
Ⅱ. MATERIALS AND METHODS 5
2.1 Expression and Purification of hTREM2174 5
2.2 Proteolytic digestion of hTREM2174 6
2.3 Immunization and hybridoma fusion 6
2.4 ELISA (Enzyme-linked immunosorbent assay) 7
2.5 Purification of TREM2 monoclonal antibody 8
2.6 Sequence determination of TREM2 monoclonal antibodies 8
2.7 Mouse genotyping 10
Ⅲ. RESULTS 12
3.1 Purification of TREM2 ectodomain 12
3.2 Antibody generation against hTREM2 ectodomain 17
3.3 Antibody subtyping and production 21
3.4 Genotyping of the hTREM2 knock-in mouse 27
Ⅳ. DISCUSSION 30
Ⅴ. REFERENCES 33
Ⅵ. Abstract in Korea (요약문) 35

URI

http://hdl.handle.net/20.500.11750/45701
http://dgist.dcollection.net/common/orgView/200000658331

DOI

10.22677/THESIS.200000658331

Degree

Master

Department

Department of Brain Sciences

Publisher

DGIST

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