Investigation of the protective role of Glutaredoxin 1 in the tauopathy through inhibiting aggregation of tau
- Title
- Investigation of the protective role of Glutaredoxin 1 in the tauopathy through inhibiting aggregation of tau
- Alternative Title
- 타우 응집 억제를 통한 Glutaredoxin 1의 타우병증에서 보호적 역할 규명
- Author(s)
- Eunmi Lee
- DGIST Authors
- Eunmi Lee ; Sung Bae Lee ; Jaekwang Kim
- Advisor
- 이성배
- Co-Advisor(s)
- Jaekwang Kim
- Issued Date
- 2023
- Awarded Date
- 2023-02-01
- Type
- Thesis
- Description
- Alzheimer's disease, Tauopathy, Glutaredoxin 1, Templated aggregation of tau, Reactive oxygen species, Apoptosis, Autophagy
- Table Of Contents
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Ⅰ. Introduction 1
Ⅱ. Material and Methods 4
2.1 Cell culture 4
2.2 Triton X-100 fraction assay 4
2.3 Immunoprecipitation assay 5
2.4 Western blot 6
2.5 Thioflavin S staining 7
2.6 Immunocytochemical staining 8
2.7 RNA isolation and Quantitative Real Time Polymerase Chain Reaction (qRT PCR) 8
2.8 Mutagenesis of Cysteine 9
2.9 ROS assay 10
2.10 Glutaredoxin fluorometric activity assay 10
2.11 In vitro recombinant tau glutathionylation assay 11
2.12 TEM imaging 11
2.13 Statistical analysis 12
Ⅲ. Results 13
3.1 Development of a cellular model of templated aggregation of tau 13
3.2 Overexpression of GRX1 reduces the levels of soluble and insoluble tau in a cellular model of templated aggregation of tau 18
3.3 Downregulation of GRX1 increases the levels of soluble and insoluble tau in a cellular model of templated aggregation of tau 23
3.4 GRX1 regulates the ROS levels in a cellular model of templated aggregation of tau 26
3.5 Overexpression of GRX1 inhibits apoptosis in a cellular model of templated aggregation of tau 29
3.6 Tau is not occurred glutathionylation in cells 32
3.7 GRX1 may accelerate the degration pathway of P301S tau by autophagic lysosomal pathway 38
Ⅳ. Discussion 42
Ⅴ. Reference 45
Ⅵ. Abstract in Korean 50
- URI
-
http://hdl.handle.net/20.500.11750/45780
http://dgist.dcollection.net/common/orgView/200000658141
- Degree
- Master
- Department
- Department of Brain Sciences
- Publisher
- DGIST
- Related Researcher
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Lee, Sung Bae
- Research Interests Cellular mechanism of neurodegenerative diseases; Neuronal maintenance and remodeling; 퇴행성 뇌질환의 세포기전; 신경계 유지 및 리모델링 연구
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- Files in This Item:
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- Appears in Collections:
- Department of Brain Sciences Theses Master
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