Title

Investigation of the protective role of Glutaredoxin 1 in the tauopathy through inhibiting aggregation of tau

Alternative Title

타우 응집 억제를 통한 Glutaredoxin 1의 타우병증에서 보호적 역할 규명

DGIST Authors

Eunmi Lee ; Sung Bae Lee ; Jaekwang Kim

Advisor

이성배

Co-Advisor(s)

Jaekwang Kim

Issued Date

2023

Awarded Date

2023-02-01

Citation

Eunmi Lee. (2023). Investigation of the protective role of Glutaredoxin 1 in the tauopathy through inhibiting aggregation of tau. doi: 10.22677/THESIS.200000658141

Type

Thesis

Description

Alzheimer's disease, Tauopathy, Glutaredoxin 1, Templated aggregation of tau, Reactive oxygen species, Apoptosis, Autophagy

Table Of Contents

Ⅰ. Introduction 1
Ⅱ. Material and Methods 4
2.1 Cell culture 4
2.2 Triton X-100 fraction assay 4
2.3 Immunoprecipitation assay 5
2.4 Western blot 6
2.5 Thioflavin S staining 7
2.6 Immunocytochemical staining 8
2.7 RNA isolation and Quantitative Real Time Polymerase Chain Reaction (qRT PCR) 8
2.8 Mutagenesis of Cysteine 9
2.9 ROS assay 10
2.10 Glutaredoxin fluorometric activity assay 10
2.11 In vitro recombinant tau glutathionylation assay 11
2.12 TEM imaging 11
2.13 Statistical analysis 12
Ⅲ. Results 13
3.1 Development of a cellular model of templated aggregation of tau 13
3.2 Overexpression of GRX1 reduces the levels of soluble and insoluble tau in a cellular model of templated aggregation of tau 18
3.3 Downregulation of GRX1 increases the levels of soluble and insoluble tau in a cellular model of templated aggregation of tau 23
3.4 GRX1 regulates the ROS levels in a cellular model of templated aggregation of tau 26
3.5 Overexpression of GRX1 inhibits apoptosis in a cellular model of templated aggregation of tau 29
3.6 Tau is not occurred glutathionylation in cells 32
3.7 GRX1 may accelerate the degration pathway of P301S tau by autophagic lysosomal pathway 38
Ⅳ. Discussion 42
Ⅴ. Reference 45
Ⅵ. Abstract in Korean 50

URI

http://hdl.handle.net/20.500.11750/45780
http://dgist.dcollection.net/common/orgView/200000658141

DOI

10.22677/THESIS.200000658141

Degree

Master

Department

Department of Brain Sciences

Publisher

DGIST

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