Title

Structural and functional characterization of a hyperpolarization-activated Cl- channel, CLC-2

DGIST Authors

Hyeon Seong Jeong ; Kyuhyung Kim ; Hyun-Ho Lim

Advisor

김규형

Co-Advisor(s)

Hyun-Ho Lim

Issued Date

2023

Awarded Date

2023-08-01

Citation

Hyeon Seong Jeong. (2023). Structural and functional characterization of a hyperpolarization-activated Cl- channel, CLC-2. doi: 10.22677/THESIS.200000689187

Type

Thesis

Description

CLC channel; hyperpolarization-activated chloride channel; voltage dependency; whole-cell-patch-clamp; Cryo-EM

Table Of Contents

1. INTRODUCTION 1
2. MATERIALS AND METHODS 6
2.1 Plasmid DNA construction 6
2.2 Electrophysiological recording 6
2.3 Western blot analysis 7
2.4 Protein expression and purification 8
2.5 Nano-disc reconstitution 9
2.6 Negative staining electron microscopy (EM) 10
2.7 Cryo-EM data collection and processing 10
3. RESULTS 13
3.1 Electrophysiological characterization of mCLC-2 wild-type 13
3.2 Electrophysiological characterization of mCLC-2 mutants 15
3.3 Detergent screening and purification of mCLC-2 22
3.4 Determination of structure of mCLC-2 using microscope 29
4. DISCUSSION 36
5. REFERENCES 41
6. Abstract in Korea (요약문) 45

URI

http://hdl.handle.net/20.500.11750/46421
http://dgist.dcollection.net/common/orgView/200000689187

DOI

10.22677/THESIS.200000689187

Degree

Master

Department

Department of Brain Sciences

Publisher

DGIST

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