Structural and functional characterization of a hyperpolarization-activated Cl- channel, CLC-2
- Title
- Structural and functional characterization of a hyperpolarization-activated Cl- channel, CLC-2
- Author(s)
- Hyeon Seong Jeong
- DGIST Authors
- Hyeon Seong Jeong ; Kyuhyung Kim ; Hyun-Ho Lim
- Advisor
- 김규형
- Co-Advisor(s)
- Hyun-Ho Lim
- Issued Date
- 2023
- Awarded Date
- 2023-08-01
- Type
- Thesis
- Description
- CLC channel; hyperpolarization-activated chloride channel; voltage dependency; whole-cell-patch-clamp; Cryo-EM
- Table Of Contents
-
1. INTRODUCTION 1
2. MATERIALS AND METHODS 6
2.1 Plasmid DNA construction 6
2.2 Electrophysiological recording 6
2.3 Western blot analysis 7
2.4 Protein expression and purification 8
2.5 Nano-disc reconstitution 9
2.6 Negative staining electron microscopy (EM) 10
2.7 Cryo-EM data collection and processing 10
3. RESULTS 13
3.1 Electrophysiological characterization of mCLC-2 wild-type 13
3.2 Electrophysiological characterization of mCLC-2 mutants 15
3.3 Detergent screening and purification of mCLC-2 22
3.4 Determination of structure of mCLC-2 using microscope 29
4. DISCUSSION 36
5. REFERENCES 41
6. Abstract in Korea (요약문) 45
- URI
-
http://hdl.handle.net/20.500.11750/46421
http://dgist.dcollection.net/common/orgView/200000689187
- Degree
- Master
- Department
- Department of Brain Sciences
- Publisher
- DGIST
- Related Researcher
-
-
Kim, Kyuhyung
- Research Interests Neurobehavior; Neural Circuit; Neurodevelopment; 신경회로; 신경행동; 신경발생; 신경유전학
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- Files in This Item:
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- Appears in Collections:
- Department of Brain Sciences Theses Master
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