Full metadata record
| DC Field | Value | Language |
|---|---|---|
| dc.contributor.advisor | 김규형 | - |
| dc.contributor.author | Hyeon Seong Jeong | - |
| dc.date.accessioned | 2023-09-18T21:00:58Z | - |
| dc.date.available | 2023-09-18T21:00:58Z | - |
| dc.date.issued | 2023 | - |
| dc.identifier.uri | http://hdl.handle.net/20.500.11750/46421 | - |
| dc.identifier.uri | http://dgist.dcollection.net/common/orgView/200000689187 | - |
| dc.description | CLC channel; hyperpolarization-activated chloride channel; voltage dependency; whole-cell-patch-clamp; Cryo-EM | - |
| dc.description.tableofcontents | 1. INTRODUCTION 1 2. MATERIALS AND METHODS 6 2.1 Plasmid DNA construction 6 2.2 Electrophysiological recording 6 2.3 Western blot analysis 7 2.4 Protein expression and purification 8 2.5 Nano-disc reconstitution 9 2.6 Negative staining electron microscopy (EM) 10 2.7 Cryo-EM data collection and processing 10 3. RESULTS 13 3.1 Electrophysiological characterization of mCLC-2 wild-type 13 3.2 Electrophysiological characterization of mCLC-2 mutants 15 3.3 Detergent screening and purification of mCLC-2 22 3.4 Determination of structure of mCLC-2 using microscope 29 4. DISCUSSION 36 5. REFERENCES 41 6. Abstract in Korea (요약문) 45 |
- |
| dc.format.extent | 45 | - |
| dc.language | eng | - |
| dc.publisher | DGIST | - |
| dc.title | Structural and functional characterization of a hyperpolarization-activated Cl- channel, CLC-2 | - |
| dc.type | Thesis | - |
| dc.identifier.doi | 10.22677/THESIS.200000689187 | - |
| dc.description.degree | Master | - |
| dc.contributor.department | Department of Brain Sciences | - |
| dc.contributor.coadvisor | Hyun-Ho Lim | - |
| dc.date.awarded | 2023-08-01 | - |
| dc.publisher.location | Daegu | - |
| dc.description.database | dCollection | - |
| dc.citation | XT.BM 정94 202308 | - |
| dc.date.accepted | 2023-09-14 | - |
| dc.contributor.alternativeDepartment | 뇌과학과 | - |
| dc.subject.keyword | CLC channel | - |
| dc.subject.keyword | hyperpolarization-activated chloride channel | - |
| dc.subject.keyword | voltage dependency | - |
| dc.subject.keyword | whole-cell-patch-clamp | - |
| dc.subject.keyword | Cryo-EM | - |
| dc.contributor.affiliatedAuthor | Hyeon Seong Jeong | - |
| dc.contributor.affiliatedAuthor | Kyuhyung Kim | - |
| dc.contributor.affiliatedAuthor | Hyun-Ho Lim | - |
| dc.contributor.alternativeName | 정현성 | - |
| dc.contributor.alternativeName | Kyuhyung Kim | - |
| dc.contributor.alternativeName | 임현호 | - |
| dc.rights.embargoReleaseDate | 2025-08-31 | - |
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