Hyeon Seong Jeong. (2023). Structural and functional characterization of a hyperpolarization-activated Cl- channel, CLC-2. doi: 10.22677/THESIS.200000689187
Type
Thesis
Description
CLC channel; hyperpolarization-activated chloride channel; voltage dependency; whole-cell-patch-clamp; Cryo-EM
Table Of Contents
1. INTRODUCTION 1 2. MATERIALS AND METHODS 6 2.1 Plasmid DNA construction 6 2.2 Electrophysiological recording 6 2.3 Western blot analysis 7 2.4 Protein expression and purification 8 2.5 Nano-disc reconstitution 9 2.6 Negative staining electron microscopy (EM) 10 2.7 Cryo-EM data collection and processing 10 3. RESULTS 13 3.1 Electrophysiological characterization of mCLC-2 wild-type 13 3.2 Electrophysiological characterization of mCLC-2 mutants 15 3.3 Detergent screening and purification of mCLC-2 22 3.4 Determination of structure of mCLC-2 using microscope 29 4. DISCUSSION 36 5. REFERENCES 41 6. Abstract in Korea (요약문) 45
