Full metadata record
| DC Field | Value | Language |
|---|---|---|
| dc.contributor.advisor | 김유리 | - |
| dc.contributor.author | Hyogyung Shin | - |
| dc.date.accessioned | 2023-09-18T21:00:59Z | - |
| dc.date.available | 2023-09-18T21:00:59Z | - |
| dc.date.issued | 2023 | - |
| dc.identifier.uri | http://hdl.handle.net/20.500.11750/46423 | - |
| dc.identifier.uri | http://dgist.dcollection.net/common/orgView/200000686904 | - |
| dc.description | Clostridium perfringens; endospore; small acid-soluble protein; DNA protection; sortase-mediated fluorescent labeling | - |
| dc.description.tableofcontents | Ⅰ. Introduction 1 Ⅱ. Materials and Methods 9 2.1 Wild-type Ssp4 expression plasmids 9 2.2 Purification of Ssp4 9 2.3 Preparation of DNA 10 2.4 Electrophoretic mobility shift assay (EMSA) 11 2.5 DNA protection assay against UV irradiation 12 2.6 DNA protection assay against DNase I degradation 13 2.7 Site-directed mutagenesis for N-terminal GGG-Ssp4 14 2.8 Purification of sortase A 15 Ⅲ. Results 17 3.1 Purification of Ssp4:D36 and Ssp4:G36 proteins 17 3.2 DNA binding affinity for Ssp4 variants for random-sequence DNA 20 3.3 DNA binding affinity for Ssp4 variants for AT-rich DNA 24 3.4 DNA protection activity of Ssp4 variants 27 3.5 Sortase-mediated fluorescent labeling 31 3.5.1 Construction of N-terminal GGG-Ssp4 vector 31 3.5.2 Purification of sortase A 34 3.5.3 Establishment of sortase-mediated fluorescent labeling strategy for Ssp4 36 Ⅳ. Discussion 40 References 44 Summary in Korean 56 |
- |
| dc.format.extent | 56 | - |
| dc.language | eng | - |
| dc.publisher | DGIST | - |
| dc.title | Characterization of Endospore Proteins Associated with DNA Protection | - |
| dc.title.alternative | 디옥시리보핵산 보호와 관련된 내생포자 단백질의 특성 분석 | - |
| dc.type | Thesis | - |
| dc.identifier.doi | 10.22677/THESIS.200000686904 | - |
| dc.description.degree | Master | - |
| dc.contributor.department | Department of New Biology | - |
| dc.contributor.coadvisor | Young-Sam Lee | - |
| dc.date.awarded | 2023-08-01 | - |
| dc.publisher.location | Daegu | - |
| dc.description.database | dCollection | - |
| dc.citation | XT.NM 신95 202308 | - |
| dc.date.accepted | 2023-09-14 | - |
| dc.contributor.alternativeDepartment | 뉴바이올로지학과 | - |
| dc.subject.keyword | Clostridium perfringens | - |
| dc.subject.keyword | endospore | - |
| dc.subject.keyword | small acid-soluble protein | - |
| dc.subject.keyword | DNA protection | - |
| dc.subject.keyword | sortase-mediated fluorescent labeling | - |
| dc.contributor.affiliatedAuthor | Hyogyung Shin | - |
| dc.contributor.affiliatedAuthor | Yoori Kim | - |
| dc.contributor.affiliatedAuthor | Young-Sam Lee | - |
| dc.contributor.alternativeName | 신효경 | - |
| dc.contributor.alternativeName | Yoori Kim | - |
| dc.contributor.alternativeName | 이영삼 | - |
| dc.rights.embargoReleaseDate | 2026-08-31 | - |
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