Profiling the biofouling characteristics of pathogenic microorganisms and developing an orthopedic implant with suppressed biofilm formation
- Title
- Profiling the biofouling characteristics of pathogenic microorganisms and developing an orthopedic implant with suppressed biofilm formation
- Author(s)
- Yoo-Jin Roh
- DGIST Authors
- Yoo-Jin Roh ; Chang-Hun Lee ; Jung-Ah Cho
- Advisor
- 이창훈
- Co-Advisor(s)
- Jung-Ah Cho
- Issued Date
- 2023
- Awarded Date
- 2023-08-01
- Type
- Thesis
- Description
- 미생물; 생물막; 생물막 형성 억제; 에피갈로카테킨 갈레이트; 티타늄 임플란트
- Table Of Contents
-
I. Introduction 1
II. Material and methods 6
1. In vitro 6
1.1 Culture of microorganisms 6
1.2 Congo red test 6
1.3 Measurement of bacterial growth 7
1.4 Bacterial Adhesion to Hydrocarbon test (BATH) 8
1.5 Biofilm formation on a solid surface and Crystal Violet (CV) assay 8
1.6 Test for the effect of Epigallro Catechine Gallate (EGCG) 10
1.7 Coating titanium implant with EGCG-containing polymer compound, ABINS 11
1.8 Cell adhesion and cytotoxicity test on ABINS 11
1.9 Biofilm formation and quantification on ABINS 12
2. In vivo 13
2.1 Surgery of rabbit femoral fracture by using ABINS 13
2.2 Sample preparation for histological staining 13
2.3 Hematoxylin and eosin (H&E) staining 14
2.4 Masson’s trichrome (MT) staining 14
2.5 Immunohistochemistry (IHC) staining 15
2.6 Western blot 15
2.7 Tartrate-Resistant Acid Phosphatase (TRAP) staining 16
3. Statistical analysis 16
III. Results 18
1. Bacterial growth rate and hydrophobicity were not primary determinants of bacterial bio-film-forming ability 18
1.1 Determining that the bacteria used in the experiment are biofilm producers 18
1.2 Measuring the growth rate of each bacterium 18
1.3 Measuring the hydrophobicity of each bacterium 19
1.4 Assessing the time kinetics of bacterial biofilm formation 19
2. The preferred solid surface for biofilm formation is different according to bacteria 24
3. Treatment of EGCG solution suppress the biofilm formation of some microorganisms 27
3.1 EGCG affect the bacterial growth, hydrophobicity, and biofilm-forming ability 27
3.2 Evaluating changes in biofilm-forming abilities on polystyrene and titanium surfaces by adding 1mM of EGCG 27
4. Titanium implant coated with ABINS shows an anti-biofouling effect in vivo 30
4.1 Confirming the cell adhesion, cytotoxicity, and anti-biofouling effect of the titanium im-plant coated with ABINS 30
4.2 Comparison of surgical results when using conventional titanium implant and using ABINS through histological staining, western blot, and TRAP assay 33
IV. Discussion 39
V. Conclusion 44
VI. References 45
- URI
-
http://hdl.handle.net/20.500.11750/46425
http://dgist.dcollection.net/common/orgView/200000684275
- Degree
- Master
- Department
- Department of New Biology
- Publisher
- DGIST
- Related Researcher
-
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Lee, Chang-Hun
- Research Interests Structure-Function relationship of cytoskeletal proteins and membrane proteins; Structure-based design of biomolecules and drugs; Development of drug delivery system in skin
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- Appears in Collections:
- Department of New Biology Theses Master
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