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Transcriptomic Analysis Reveals Wound Healing of Morus alba Root Extract by Up-Regulating Keratin Filament and CXCL12/CXCR4 Signaling

Transcriptomic Analysis Reveals Wound Healing of Morus alba Root Extract by Up-Regulating Keratin Filament and CXCL12/CXCR4 Signaling
Kim, Kang-HoonChung, Won-SeokKim, YoomiKim, Ki-SukLee, In-SeungPark, Ji YoungJeong, Hyeon-SooNa, Yun-CheolLee, Chang-HunJang, Hyeung-Jin
DGIST Authors
Lee, Chang-Hun
Issue Date
Phytotherapy Research, 29(8), 1251-1258
Article Type
AnimalAnimal CellAnimal ExperimentAnimal ModelAnimalsApoptosisC-X-C Motif Chemokine 12 (CXCL12)Cell CycleCell DifferentiationCell GrowthCell LineCell MigrationCell ProliferationCell ViabilityChemistryChemokine CXCL12Chemokine Receptor 4 (CXCR4)Chemokine Receptor CXCR4Controlled StudyCXCL12 Protein, MouseCXCR4 Protein, MouseCytokeratin 14Cytokeratin 6Cytokeratin 6ACytologyDown-RegulationDrug EffectsDrug EfficacyDrug MechanismExtracellular MatrixImmune ResponseIn Vitro StudyIn Vitro TechniquesInstitute for Cancer Research MouseKeratinKeratinocyteKeratinocytesKeratinsLipid MetabolismMessenger RNAMetabolismMice, Inbred ICRMorusMorus AlbaMorus Alba RootMouseMulberry ExtractNon-HumanPlant ExtractPlant ExtractsPlant RootPlant RootsPrimary Cell CultureProtein ExpressionProtein FunctionReceptors, CXCR4RNA, MessengerSignal TransductionSkinStromal Cell Derived Factor 1Therapeutic TreatmentTranscriptomeUnclassified DrugUp-RegulationUpregulationWound Healing
Facilitation of the wound healing process is important because a prolonged wound site increases pain and the risk of infection. In oriental medicine, an extract of Morus alba root (MA) has usually been prescribed as traditional treatment for accelerating wound healing, and it has been proven to be safe for centuries. To study the molecular mechanism of MA-mediated skin wound healing, we performed a primary cell culture and a skin explant culture and observed significant difference between the groups with and without MA extract. In the cellular system, a real-time cell analysis and real-time quantitative PCR were performed. It was found that MA extract enhanced proliferation in a dose-dependent manner on Kera-308 cell line, and up-regulated keratin expression including wound-induced Krt6a. In skin explant culture, the mRNA level derived from cell outgrowth displayed a tendency toward more up-regulated mRNA associated keratin filaments and toward a more up-regulated mRNA level of C-X-C motif chemokine 12 (CXCL12) and a chemokine receptor 4 (CXCR4) axis signaling pathway downstream. In this process, we concluded that MA extract had a scientific possibility of wound repair by increasing intracellular and extracellular supports and by inducing a CXCL12/CXCR4 signaling pathway. © 2015 John Wiley & Sons, Ltd.
Wiley Blackwell
Related Researcher
  • Author Lee, Chang-Hun  
  • Research Interests Structure-Function relationship of cytoskeletal proteins and membrane proteins; Structure-based design of biomolecules and drugs; Development of drug delivery system in skin
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