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Transcriptomic Analysis Reveals Wound Healing of Morus alba Root Extract by Up-Regulating Keratin Filament and CXCL12/CXCR4 Signaling
- Transcriptomic Analysis Reveals Wound Healing of Morus alba Root Extract by Up-Regulating Keratin Filament and CXCL12/CXCR4 Signaling
- Kim, Kang-Hoon; Chung, Won-Seok; Kim, Yoomi; Kim, Ki-Suk; Lee, In-Seung; Park, Ji Young; Jeong, Hyeon-Soo; Na, Yun-Cheol; Lee, Chang-Hun; Jang, Hyeung-Jin
- DGIST Authors
- Lee, Chang-Hun
- Issue Date
- Phytotherapy Research, 29(8), 1251-1258
- Article Type
- Animal; Animal Cell; Animal Experiment; Animal Model; Animals; Apoptosis; C-X-C Motif Chemokine 12 (CXCL12); Cell Cycle; Cell Differentiation; Cell Growth; Cell Line; Cell Migration; Cell Proliferation; Cell Viability; Chemistry; Chemokine CXCL12; Chemokine Receptor 4 (CXCR4); Chemokine Receptor CXCR4; Controlled Study; CXCL12 Protein, Mouse; CXCR4 Protein, Mouse; Cytokeratin 14; Cytokeratin 6; Cytokeratin 6A; Cytology; Down-Regulation; Drug Effects; Drug Efficacy; Drug Mechanism; Extracellular Matrix; Immune Response; In Vitro Study; In Vitro Techniques; Institute for Cancer Research Mouse; Keratin; Keratinocyte; Keratinocytes; Keratins; Lipid Metabolism; Messenger RNA; Metabolism; Mice, Inbred ICR; Morus; Morus Alba; Morus Alba Root; Mouse; Mulberry Extract; Non-Human; Plant Extract; Plant Extracts; Plant Root; Plant Roots; Primary Cell Culture; Protein Expression; Protein Function; Receptors, CXCR4; RNA, Messenger; Signal Transduction; Skin; Stromal Cell Derived Factor 1; Therapeutic Treatment; Transcriptome; Unclassified Drug; Up-Regulation; Upregulation; Wound Healing
- Facilitation of the wound healing process is important because a prolonged wound site increases pain and the risk of infection. In oriental medicine, an extract of Morus alba root (MA) has usually been prescribed as traditional treatment for accelerating wound healing, and it has been proven to be safe for centuries. To study the molecular mechanism of MA-mediated skin wound healing, we performed a primary cell culture and a skin explant culture and observed significant difference between the groups with and without MA extract. In the cellular system, a real-time cell analysis and real-time quantitative PCR were performed. It was found that MA extract enhanced proliferation in a dose-dependent manner on Kera-308 cell line, and up-regulated keratin expression including wound-induced Krt6a. In skin explant culture, the mRNA level derived from cell outgrowth displayed a tendency toward more up-regulated mRNA associated keratin filaments and toward a more up-regulated mRNA level of C-X-C motif chemokine 12 (CXCL12) and a chemokine receptor 4 (CXCR4) axis signaling pathway downstream. In this process, we concluded that MA extract had a scientific possibility of wound repair by increasing intracellular and extracellular supports and by inducing a CXCL12/CXCR4 signaling pathway. © 2015 John Wiley & Sons, Ltd.
- Wiley Blackwell
- Related Researcher
Structure-Function relationship of cytoskeletal proteins and membrane proteins; Structure-based design of biomolecules and drugs; Development of drug delivery system in skin
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