Full metadata record
DC Field | Value | Language |
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dc.contributor.author | Park, Hye-Jung | - |
dc.contributor.author | Kim, Sang Kyu | - |
dc.contributor.author | Park, Kyoungsook | - |
dc.contributor.author | Lyu, Hong-Kun | - |
dc.contributor.author | Lee, Chang-Soo | - |
dc.contributor.author | Chung, Sang J. | - |
dc.contributor.author | Yun, Wan Soo | - |
dc.contributor.author | Kim, Moonil | - |
dc.contributor.author | Chung, Bong Hyun | - |
dc.date.accessioned | 2018-01-25T01:17:07Z | - |
dc.date.available | 2018-01-25T01:17:07Z | - |
dc.date.created | 2017-04-10 | - |
dc.date.issued | 2009-01-05 | - |
dc.identifier.issn | 0014-5793 | - |
dc.identifier.uri | http://hdl.handle.net/20.500.11750/5453 | - |
dc.description.abstract | Here we describe an ion sensitive field effect transistor (ISFET) biosensor, which was designed to monitor directly the surface charge of structurally altered maltose binding protein (MBP) upon stimulation with maltose. This study is the first report of the application of a FET biosensor to the monitoring of conformationally changed proteins. Consequently, a significant drop in current on the basis of the charge-dependent capacitance measurement has been clearly observed in response to maltose, but not for the glucose control, thereby indicating that the substrate-specific conformational properties of the target protein could be successfully monitored using the ISFET. Collectively, our results clearly suggest that ISFET provide a high fidelity system for the detection of maltose-induced structural alterations in MBP. © 2008 Federation of European Biochemical Societies. | - |
dc.publisher | Wiley Oxford office | - |
dc.title | An ISFET biosensor for the monitoring of maltose-induced conformational changes in MBP | - |
dc.type | Article | - |
dc.identifier.doi | 10.1016/j.febslet.2008.11.039 | - |
dc.identifier.wosid | 000261949200028 | - |
dc.identifier.scopusid | 2-s2.0-57749210425 | - |
dc.identifier.bibliographicCitation | FEBS Letters, v.583, no.1, pp.157 - 162 | - |
dc.subject.keywordAuthor | ISFET | - |
dc.subject.keywordAuthor | Biosensor | - |
dc.subject.keywordAuthor | Surface charge | - |
dc.subject.keywordAuthor | Conformational change | - |
dc.subject.keywordAuthor | MBP | - |
dc.subject.keywordPlus | Article | - |
dc.subject.keywordPlus | BINDING PROTEIN | - |
dc.subject.keywordPlus | Biological Monitoring | - |
dc.subject.keywordPlus | Biosensing Techniques | - |
dc.subject.keywordPlus | Biosensor | - |
dc.subject.keywordPlus | Carrier Proteins | - |
dc.subject.keywordPlus | CELLS | - |
dc.subject.keywordPlus | CHARGE | - |
dc.subject.keywordPlus | Conformational Change | - |
dc.subject.keywordPlus | Conformational Transition | - |
dc.subject.keywordPlus | DNA | - |
dc.subject.keywordPlus | FIELD-EFFECT TRANSISTOR | - |
dc.subject.keywordPlus | Ion Sensitive Field Effect Transistor | - |
dc.subject.keywordPlus | ISFET | - |
dc.subject.keywordPlus | Maltose | - |
dc.subject.keywordPlus | Maltose Binding Protein | - |
dc.subject.keywordPlus | MBP | - |
dc.subject.keywordPlus | Priority Journal | - |
dc.subject.keywordPlus | Protein Conformation | - |
dc.subject.keywordPlus | SemICONDUCTORS | - |
dc.subject.keywordPlus | SENSOR | - |
dc.subject.keywordPlus | SURFACE | - |
dc.subject.keywordPlus | Surface Charge | - |
dc.citation.endPage | 162 | - |
dc.citation.number | 1 | - |
dc.citation.startPage | 157 | - |
dc.citation.title | FEBS Letters | - |
dc.citation.volume | 583 | - |
dc.description.journalRegisteredClass | scopus | - |
dc.relation.journalResearchArea | Biochemistry & Molecular Biology; Biophysics; Cell Biology | - |
dc.relation.journalWebOfScienceCategory | Biochemistry & Molecular Biology; Biophysics; Cell Biology | - |
dc.type.docType | Article | - |
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