Full metadata record
DC Field | Value | Language |
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dc.contributor.author | Naik, Mandar T | - |
dc.contributor.author | Kang, Moo Seok | - |
dc.contributor.author | Ho, ChunChen | - |
dc.contributor.author | Liao, Pei Hsin | - |
dc.contributor.author | Hsieh, Yung-Lin | - |
dc.contributor.author | Naik, Nandita M | - |
dc.contributor.author | Wang, Szu Huan | - |
dc.contributor.author | Chang, Ik Soo | - |
dc.contributor.author | Shih, Hsiu Ming | - |
dc.contributor.author | Huang, Tai Huang | - |
dc.date.accessioned | 2018-02-05T04:12:03Z | - |
dc.date.available | 2018-02-05T04:12:03Z | - |
dc.date.created | 2018-01-18 | - |
dc.date.issued | 2017-12 | - |
dc.identifier.citation | Scientific Reports, v.7, no.1 | - |
dc.identifier.issn | 2045-2322 | - |
dc.identifier.uri | http://hdl.handle.net/20.500.11750/5625 | - |
dc.description.abstract | The negatively charged amino acid-dependent sumoylation motif (NDSM) carries an additional stretch of acidic residues downstream of the consensus Ψ-K-x-E/D sumoylation motif. We have previously shown that acetylation of the SUMO E2 conjugase enzyme, Ubc9, at K65 downregulates its binding to the NDSM and renders a selective decrease in sumoylation of substrates with the NDSM motif. Here, we provide detailed structural, thermodynamic, and kinetics results of the interactions between Ubc9 and its K65 acetylated variant (Ac-Ubc9K65) with three NDSMs derived from Elk1, CBP, and Calpain2 to rationalize the mechanism beneath this reduced binding. Our nuclear magnetic resonance (NMR) data rule out a direct interaction between the NDSM and the K65 residue of Ubc9. Similarly, we found that NDSM binding was entropy-driven and unlikely to be affected by the negative charge by K65 acetylation. Moreover our NMR, mutagenesis and molecular dynamics simulation studies defined the sequence of the NDSM as Ψ-K-x-E/D-x1-x2-(x3/E/D)-(x4/E/D)-xn and determined that K74 and K76 were critical Ubc9 residues interacting with the negatively charged residues of the NDSM. © 2017 The Author(s). | - |
dc.language | English | - |
dc.publisher | Nature Publishing Group | - |
dc.title | Molecular mechanism of K65 acetylation-induced attenuation of Ubc9 and the NDSM interaction | - |
dc.type | Article | - |
dc.identifier.doi | 10.1038/s41598-017-17465-0 | - |
dc.identifier.wosid | 000417689400015 | - |
dc.identifier.scopusid | 2-s2.0-85037722627 | - |
dc.type.local | Article(Overseas) | - |
dc.type.rims | ART | - |
dc.description.journalClass | 1 | - |
dc.citation.publicationname | Scientific Reports | - |
dc.contributor.nonIdAuthor | Naik, Mandar T | - |
dc.contributor.nonIdAuthor | Kang, Moo Seok | - |
dc.contributor.nonIdAuthor | Ho, ChunChen | - |
dc.contributor.nonIdAuthor | Liao, Pei Hsin | - |
dc.contributor.nonIdAuthor | Hsieh, Yung-Lin | - |
dc.contributor.nonIdAuthor | Naik, Nandita M | - |
dc.contributor.nonIdAuthor | Wang, Szu Huan | - |
dc.contributor.nonIdAuthor | Shih, Hsiu Ming | - |
dc.contributor.nonIdAuthor | Huang, Tai Huang | - |
dc.identifier.citationVolume | 7 | - |
dc.identifier.citationNumber | 1 | - |
dc.identifier.citationTitle | Scientific Reports | - |
dc.type.journalArticle | Article | - |
dc.description.isOpenAccess | Y | - |
dc.subject.keywordPlus | UBIQUITIN-CONJUGATING ENZYME | - |
dc.subject.keywordPlus | PROTEIN-PROTEIN DOCKING | - |
dc.subject.keywordPlus | SUMO CONJUGATION | - |
dc.subject.keywordPlus | E3 LIGASE | - |
dc.subject.keywordPlus | SITE | - |
dc.subject.keywordPlus | SPECIFICITY | - |
dc.subject.keywordPlus | COMPLEX | - |
dc.subject.keywordPlus | BINDING | - |
dc.subject.keywordPlus | E2 | - |
dc.subject.keywordPlus | PHOSPHORYLATION | - |
dc.contributor.affiliatedAuthor | Naik, Mandar T | - |
dc.contributor.affiliatedAuthor | Kang, Moo Seok | - |
dc.contributor.affiliatedAuthor | Ho, ChunChen | - |
dc.contributor.affiliatedAuthor | Liao, Pei Hsin | - |
dc.contributor.affiliatedAuthor | Hsieh, Yung-Lin | - |
dc.contributor.affiliatedAuthor | Naik, Nandita M | - |
dc.contributor.affiliatedAuthor | Wang, Szu Huan | - |
dc.contributor.affiliatedAuthor | Chang, Ik Soo | - |
dc.contributor.affiliatedAuthor | Shih, Hsiu Ming | - |
dc.contributor.affiliatedAuthor | Huang, Tai Huang | - |
10.1038_s41598-017-17465-0.pdf
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