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Minimal information for studies of extracellular vesicles (MISEV2023): From basic to advanced approaches

Title
Minimal information for studies of extracellular vesicles (MISEV2023): From basic to advanced approaches
Author(s)
Théry, ClotildeWitwer, Kenneth W.Welsh, Joshua A.Goberdhan, Deborah C. I.O'Driscoll, LorraineBuzas, Edit I.Blenkiron, CherieBussolati, BenedettaCai, HoujianDi Vizio, DoloresDriedonks, Tom A. P.Erdbrugger, UtaFalcon-Perez, Juan M.Fu, Qing-LingHill, Andrew F.Lenassi, MetkaLim, Sai KiangMahoney, My G.Mohanty, SujataMoller, AndreasNieuwland, RienkOchiya, TakahiroSahoo, SusmitaTorrecilhas, Ana C.Zheng, LeiZijlstra, AndriesAbuelreich, SarahBagabas, ReemBergese, PaoloBridges, Esther M.Brucale, MarcoBurger, DylanCarney, Randy P.Cocucci, EmanueleColombo, FedericoCrescitelli, RossellaHanser, EdveenaHarris, Adrian L.Haughey, Norman J.Hendrix, AnIvanov, Alexander R.Jovanovic-Talisman, TijanaKruh-Garcia, Nicole A.Faustino, Vroniqa Ku'ulei-LynKyburz, DiegoLasser, CeciliaLennon, Kathleen M.Lotvall, JanMaddox, Adam L.Martens-Uzunova, Elena S.Mizenko, Rachel R.Newman, Lauren A.Ridolfi, AndreaRohde, EvaRojalin, TatuRowland, AndrewSaftics, AndrasSandau, Ursula S.Saugstad, Julie A.Shekari, FaezehSwift, SimonTer-Ovanesyan, DmitryTosar, Juan P.Useckaite, ZivileValle, FrancescoVarga, Zoltanvan der Pol, Edwinvan Herwijnen, Martijn J. C.Wauben, Marca H. M.Wehman, Ann M.Williams, SarahZendrini, AndreaZimmerman, Alan J.MISEV ConsortiumYea, KyungmooThery, Clotilde
Issued Date
2024-02
Citation
Journal of Extracellular Vesicles, v.13, no.2
Type
Article
Author Keywords
exosomesextracellular vesiclesextracellular particlesguidelinesmicroparticlesmicrovesiclesminimal information requirementsMISEVreproducibilityectosomesrigorstandardisation
Keywords
FLUID PROTEIN-CONCENTRATIONHUMAN CEREBROSPINAL-FLUIDDOUBLE-STRANDED DNAMASS-SPECTROMETRYFLOW-CYTOMETRYIN-VITROSYSTEM SUITABILITYRAMAN-SPECTROSCOPYPROTEOMIC ANALYSISMEMBRANE-VESICLES
ISSN
2001-3078
Abstract
Extracellular vesicles (EVs), through their complex cargo, can reflect the state of their cell of origin and change the functions and phenotypes of other cells. These features indicate strong biomarker and therapeutic potential and have generated broad interest, as evidenced by the steady year-on-year increase in the numbers of scientific publications about EVs. Important advances have been made in EV metrology and in understanding and applying EV biology. However, hurdles remain to realising the potential of EVs in domains ranging from basic biology to clinical applications due to challenges in EV nomenclature, separation from non-vesicular extracellular particles, characterisation and functional studies. To address the challenges and opportunities in this rapidly evolving field, the International Society for Extracellular Vesicles (ISEV) updates its ‘Minimal Information for Studies of Extracellular Vesicles’, which was first published in 2014 and then in 2018 as MISEV2014 and MISEV2018, respectively. The goal of the current document, MISEV2023, is to provide researchers with an updated snapshot of available approaches and their advantages and limitations for production, separation and characterisation of EVs from multiple sources, including cell culture, body fluids and solid tissues. In addition to presenting the latest state of the art in basic principles of EV research, this document also covers advanced techniques and approaches that are currently expanding the boundaries of the field. MISEV2023 also includes new sections on EV release and uptake and a brief discussion of in vivo approaches to study EVs. Compiling feedback from ISEV expert task forces and more than 1000 researchers, this document conveys the current state of EV research to facilitate robust scientific discoveries and move the field forward even more rapidly. © 2024 The Authors. Journal of Extracellular Vesicles published by Wiley Periodicals, LLC on behalf of the International Society for Extracellular Vesicles. This is an open access article under the terms of the Creative Commons Attribution License, which permits use, distribution and reproduction in any medium, provided the original work is properly cited.
URI
http://hdl.handle.net/20.500.11750/56696
DOI
10.1002/jev2.12404
Publisher
Wiley
Related Researcher
  • 예경무 Yea, Kyungmoo 뉴바이올로지학과
  • Research Interests Antibody; Engineering; Phage Display; Therapeutics; Immune; Exosome; Translational; Cytokine
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Appears in Collections:
Department of New Biology Bio-therapeutics Design Lab 1. Journal Articles

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