Cited 9 time in webofscience Cited 9 time in scopus

Use of Flp-mediated cassette exchange in the development of a CHO cell line stably producing erythropoietin

Title
Use of Flp-mediated cassette exchange in the development of a CHO cell line stably producing erythropoietin
Authors
Kim, Min SooLee, Gyun Min
DGIST Authors
Kim, Min Soo
Issue Date
2008-07
Citation
Journal of Microbiology and Biotechnology, 18(7), 1342-1351
Type
Article
Article Type
Article
Keywords
Flp-mediated cassette exchangestable parentalclonelong-term cultureEPO-producing CHO cell line
ISSN
1017-7825
Abstract
The feasibility of the use of Flp-mediated cassette exchange in the development of a CHO cell line, which produces erythropoietin (EPO) stably and largely, was investigated. A stable, high enhanced green fluorescence protein (EGFP)-producing clone was screened by extensive flow cytometric analysis. An EPO expression unit was targeted into the premarked locus of the stable parental clone by Flp-mediated cassette exchange and a correctly targeted clone (FC28T7) was obtained. The EPO production of FC28T7 was proven to be stable in long-term culture. Furthermore, the Flp-mediated cassette exchange did not alter the stable parental clone's characteristics concerning transgene expression level and stability. Taken together, the data obtained here indicated that the establishment of CHO cell lines stably producing a desired protein is achievable using Flp-mediated cassette exchang. © The Korean Society for Microbiology and Biotechnology.
URI
http://hdl.handle.net/20.500.11750/5832
Publisher
한국미생물·생명공학회
Related Researcher
  • Author Kim, Min Soo InfoLab
  • Research Interests Big Data Systems; Big Data Mining & Machine Learning; Big Data Bioinformatics; 데이터 마이닝 및 빅데이터 분석; 바이오인포메틱스 및 뉴로인포메틱스; 뇌-기계 인터페이스(BMI)
Files:
There are no files associated with this item.
Collection:
Department of Information and Communication EngineeringInfoLab1. Journal Articles


qrcode mendeley

Items in DSpace are protected by copyright, with all rights reserved, unless otherwise indicated.

BROWSE