The present invention relates to an Alzheimer-resistant human pluripotent stem cell model having an ApoE Christchurch mutation inserted thereinto and a construction method therefor and, more specifically, to an Alzheimer-resistant human induced pluripotent stem cell model and brain cell/brain organoid into which an ApoE Christchurch mutation is inserted using a CRISPR/Cas9 system, and a construction method therefor. According to the present invention, Alzheimer-resistant human induced pluripotent stem cells and a brain cell/brain organoid are constructed by inserting an ApoE Christchurch mutation into human induced pluripotent stem cells with the aid of a CRISPR/Cas9 gene editing technique and as such, can surmount the limitations observed in preexisting animal models, such as protein expression patterns different from those in human cells, etc. The Alzheimer-resistant cell model of the present invention can find advantageous applications in research into ApoE-associated Alzheimer pathogenesis, etc.
