Title

Regulation of KCNQ Channel Gating Properties by CaBP1

DGIST Authors

Byoungheon Kang ; Byung-Chang Suh ; Myungin Baek

Advisor

서병창

Co-Advisor(s)

Myungin Baek

Issued Date

2026

Awarded Date

2026-02-01

Type

Thesis

Description

CaBP1, CaM, KCNQ, PIP2, Myristoylation

Table Of Contents

Ⅰ. Introduction 1
II. MATERIALS AND METHODS 3
2.1 Cell culture and Transfection 3
2.2 Confocal microscopy 3
2.3 Cell lysis and Co-immunoprecipitation 3
2.4 Western blotting 4
2.5 Electrophysiological recording 4
2.6 Statistical Analysis 5
III. RESULTS 6
3.1 CaBP1 Distinctly Modulates KCNQ2/3 Current Density in Opposition to CaM 6
3.2 Voltage dependence of activation on KCNQ channel with CaM or CaBP1 6
3.3 CaBP1 Accelerates the Activation and Deactivation Kinetics of KCNQ2/3 7
3.4 N-terminal Myristoylation is the Determinant for CaBP1 Membrane Targeting 10
3.5 Membrane Localization is Prerequisite for CaBP1-Mediated Channel Regulation 10
3.6 Differential regulation of PIP2 sensitivity of KCNQ2/3 by CaBP1 vs CaM 13
3.7 CaBP1 Buffers Channel Inhibition Mediated by Physiological Gq-Protein Signaling 13
3.8 Only membrane-localized CaBP1 alters the apparent PIP₂ sensitivity of KCNQ2/3 16
3.9 CaBP1 is expressed as multiple variants across brain regions and physically associated with KCNQ2 16
3.10 Model: CaBP1 preserves KCNQ2/3 opening during partial–but not complete—PIP2 depletion at the S4-S5 linker 19
ⅠV. DISCUSSION 21
V. REFERENCES 24
VI. SUMMARY IN KOREAN 27

URI

https://scholar.dgist.ac.kr/handle/20.500.11750/59648
http://dgist.dcollection.net/common/orgView/200000947520

DOI

10.22677/THESIS.200000947520

Degree

Master

Department

Department of Brain Sciences

Publisher

DGIST

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