Title

Enhancing immunoproteasome-mediated MHC-I antigen presentation by targeting OAS1 in senescent cells

DGIST Authors

Jeonghan Lee ; Byung-Hoon Lee ; Il-Kyu Choi

Advisor

이병훈

Co-Advisor(s)

Il-Kyu Choi

Issued Date

2026

Awarded Date

2026-02-01

Type

Thesis

Description

Immunoproteasome, cellular senescence, immune surveillance, IRF1, OAS1

Table Of Contents

Ⅰ. Introduction 1
1.1 Cellular senescence 1
1.2 Immune surveillance of senescent cells 2
1.3 Immunoproteasome 5
1.4 Interferon gamma signaling in the MHC-I antigen presentation process 8
1.5 Oligoadenylate synthetase 10
1.6 Hypothesis and specific aims 13

II. Results 14
2.1 Immunoproteasome expression and activity are less induced upon IFNγ treatment in various senescent models 14
2.2 Low IRF1 protein level is attributed to reduced immunoproteasome induction in senescent cells 16
2.3 IFNγ-induced IRF1 translation is downregulated in senescent cells 18
2.4 OAS1-mediated IRF1 translation may be hindered by dsRNA and OAS3 in senescent cells 20
2.5 Surface HLA-I expression is less induced after IFNγ treatment in senescent cells 23

III. Discussion 25

IV. Materials and Methods 27
4.1 Cell culture 27
4.2 Induction of cellular senescence 27
4.3 Senescence-associated β-galactosidase staining 27
4.4 Western blot analysis 27
4.5 Proteasome activity measurement from whole cell lysates 28
4.6 RNA extraction and gene expression analysis 29
4.7 mRNA decay measurement 29
4.8 RNA immunoprecipitation 30
4.9 Immunocytochemistry 31
4.10 RNA FISH staining 31
4.11 Flow cytometry 32
4.12 Statistical analysis 33

V. References 36

URI

https://scholar.dgist.ac.kr/handle/20.500.11750/59652
http://dgist.dcollection.net/common/orgView/200000942489

DOI

10.22677/THESIS.200000942489

Degree

Master

Department

Department of New Biology

Publisher

DGIST

Show Full Item Record