Title

Temporally Distinct Postnatally Born Interneurons in the Olfactory Bulb Contribute to Functional Differentiation in Odor Processing

DGIST Authors

Jiyun Choe ; Cheil Moon ; Woong Sun

Advisor

문제일

Co-Advisor(s)

Woong Sun

Issued Date

2025

Awarded Date

2025-08-01

Type

Thesis

Description

Olfactory bulb, Postnatal neurogenesis, Interneurons (EBIs and LBIs), Odor discrimination and detection, Neuropixels recording

Table Of Contents

List of Contents
Abstract i
List of Contents ii
List of Figures v
List of Tables vi
List of Abbreviations vii

Chapter 1. General Introduction 1
1.1 The brain is a complex network of neurons, circuits, and functional areas 1
1.1.1 Structural organization 1
1.1.2 Functional integration 3
1.2 Sensory system· 7
1.2.1 General senses & Special senses 7
1.2.2 Vision, Hearing & Balance 8
1.2.3 Chemical Senses 9
1.3 Olfactory system 12
1.3.1 Odor sensing 12
1.3.2 The first processing of olfactory information in olfactory bulb 14
1.3.3 Integrated information in olfactory cortex 16
1.4 Interneurons in main olfactory bulb 19
1.4.1 Periglomerular cells 19
1.4.2 External plexiform layer interneurons 20
1.4.3 Granule cells 21
1.5 Development of interneurons in olfactory bulb 25
1.5.1 Embryonic neurogenesis 25
1.5.2 Postnatal neurogenesis 26
1.5.3 Postnatally early-born interneurons & late-born interneurons 28

Chapter 2. Materials and Methods 32
2.1 Materials 32
2.1.1 Antibodies, virus, reagents & device 32
2.2 Methods 33
2.2.1 Animals 33
2.2.2 Stereotaxic 33
2.2.3 Immunohistochemistry 33
2.3 Behavioral experiments 35
2.3.1 Preparation of behavior test 35
2.3.2 Olfactory threshold test 35
2.3.3 Olfactory habituation test 36
2.3.4 Buried food test 36
2.3.5 Olfactory Discrimination test 36
2.4 Multi-electrode recording using Neuropixels 1.0 37
2.4.1 Delivering odor cue using olfactometer 37
2.4.2 Measurement of odor concentration & mouse respiration 37
2.4.3 Acute multi-electrode recording using Neuropixels 1.0 38
2.4.4 Spike sorting for recording with Neuropixels 1.0 39

Chapter 3. Identify postnatal interneurons depends on birth timing in OB 40
3.1 Introduction 40
3.2 Result 40
3.2.1 Optimization of axis to inject adeno-associated virus 40
3.2.2 Locational differences between EBIs and LBIs 45
3.2.3 Genetically differences depending on postnatal period 50
3.3 Discussion 58

Chapter 4. Differential Behavioral Effects of Suppressing EBIs & LBIs in OB 64
4.1 Introduction 64
4.2 Result 66
4.2.1 Change of detection ability when EBIs are inhibited 66
4.2.2 Change of discrimination ability when LBIs are inhibited 71
4.2.3 Habituating behavior do not change when postnatally born interneurons
are inhibited 77
4.3 Discussion 80

Chapter 5. Observe neural activity differences when postnatally born interneurons from multi-electrode recording 84
5.1 Introduction 84
5.2 Results 86
5.2.1 Synchronization of neural activity after odor stimulation on OB 86
5.2.2 Odor concentration–dependent firing rate changes across OB layers
during inspiration and expiration phases 93
5.3 Discussion 98

Chapter 6. Conclusion 104
References 105
Abstract in Korean 120

URI

https://scholar.dgist.ac.kr/handle/20.500.11750/59754
http://dgist.dcollection.net/common/orgView/200000888289

DOI

10.22677/THESIS.200000888289

Degree

Doctor

Department

Department of Brain Sciences

Publisher

DGIST

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