Title

The EGFR and VEGFR2 inhibitor vandetanib alleviates neuroinflammation and pyroptosis through NLRP3/SOD2 and JNK/NF-kB signaling in LPS-treated wild-type mice

DGIST Authors

Ji-Yeong Jang ; Jung Ho Hyun ; Hyang-Sook Hoe

Advisor

현정호

Co-Advisor(s)

Hyang-Sook Hoe

Issued Date

2025

Awarded Date

2025-08-01

Type

Thesis

Description

Vandetanib, EGFR, VEGFR, neuroinflammation, NLRP3, JNK

Table Of Contents

List of Contents

Abstract i
List of contents ii


Ⅰ. Introduction

II. Materials and Methods
2.1 Vandetanib 3
2.2 Cell culture 3
2.3 Cytotoxicity assays 3
2.4 Wild-type mice 3
2.5 Real-time quantitative PCR 4
2.6 Nuclear fractionation 6
2.7 Western blotting 6
2.8 Immunofluorescence staining 8
2.9 Statistical analysis 9
.
III. Results
3.1 Vandetanib treatment reduces LPS-induced proinflammatory cytokine expression in BV2 microglial cells by decreasing NLRP3/SOD2 expression and JNK/NF-kB phosphorylation 10
3.2 Vandetanib suppresses proinflammatory cytokine and NLRP3/SOD2 expression and NF-kB phosphorylation in LPS-treated primary astrocytes 12
3.3 Vandetanib alleviates microgliosis and astrogliosis in LPS-treated wild-type mice 13
3.4 Vandetanib decreases LPS-evoked proinflammatory cytokine expression in wild-type mice 16
3.5 Vandetanib suppresses the expression of the neuroinflammation-associated molecular targets NLRP3 and SOD2 in LPS-treated wild-type mice 20
3.6 Vandetanib reduces LPS-mediated microglial and astroglial neuroinflammatory dynamics in WT mice 22
3.7 Vandetanib diminishes LPS-stimulated JNK/NF-kB phosphorylation in wild-type mice 23
3.8 Vandetanib alleviates LPS-mediated pyroptosis in wild-type mice 25

IV. Discussion

V. Conclusions

URI

https://scholar.dgist.ac.kr/handle/20.500.11750/59809
http://dgist.dcollection.net/common/orgView/200000893745

DOI

10.22677/THESIS.200000893745

Degree

Master

Department

Department of Brain Sciences

Publisher

DGIST

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