Title

Rapid and Convenient Detection Strategies of Extracellular Vesicles for Clinical Applications

Alternative Title

임상 응용을 위한 세포외소포의 신속하고 간편한 검출 전략

DGIST Authors

Subhra Sulipta Jena ; Seonki Hong

Advisor

홍선기

Issued Date

2026

Awarded Date

2026-08-01

Type

Thesis

Description

Extracellular vesicles, microRNA, Molecular beacons, Clinical diagnostics

Table Of Contents

List of Contents
Abstract i
List of contents ii
List of figures iv
List of Tables vii


I. INTRODUCTION 1
1.1 Background 2
1.1.1 Extracellular vesicles as biological messenger 2
1.1.2 Exosomal miRNAs as stable and informative biomarkers 2
1.1.3 Relevance of exosomal miRNAs in cardiovascular disease 2
1.1.4 Limitation of conventional exosomal miRNA detection 2
1.1.5 Direct in situ detection approaches for intact EVs 3
1.1.6 Fusion-based vesicle system for probe delivery 3
1.2 Motivation and Objective 3

II. EXPERIMENTAL SECTION 5

ⅠⅠⅠ. RESULTS AND DISCUSSION
3.1 Schematic representation of a vesicle fusion-based miRNA detection assay 10
3.2 Characterization of MB-encapsulated liposomes and fusion behavior in the SAFE
Assay 12
3.3 Comparison between conventional method with SAFE 13
3.4 Identification of cardiac injury-Related EV miRNAs 15
3.5 Verification of EV membrane Integrity after SAFE 17

3.6 Clinical validation of SAFE for diagnosing cardiovascular disease 19
3.7 Schematic representation of EV-based multiplex miRNA–protein detection 21
3.8 Relative Expression of CD63, CD24, and EpCAM in Five Colorectal Cancer
Cell Lines 23
3.9 Clinical Multiplex Analysis of Plasma EVs in Colorectal Cancer 24
3.10 Diagnostic evaluation of single and combined EV-associated biomarkers for
CRC discrimination. 26
3.11 Diagnostic performance analysis of single and combined EV-associated biomarkers
for CRC detection.28


IV. CONCLUSION 30

V. REFERENCES 31
요 약 문 34
Appendix A: List of Publication 35
Appendix B: List of Conferences 36

URI

https://scholar.dgist.ac.kr/handle/20.500.11750/60758
http://dgist.dcollection.net/common/orgView/200001006912

DOI

10.22677/THESIS.200001006912

Degree

Doctor

Department

Department of Physics and Chemistry

Publisher

DGIST

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