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  Department of Brain Sciences Theses Master

APAF1 is required for corticosterone-induced autophagic death of adult hippocampal neural stem cells

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dc.contributor.advisor 유성운 -
dc.contributor.author Yeongwon Gu -
dc.date.accessioned 2026-09-01T19:30:54Z -
dc.date.available 2026-09-01T19:30:54Z -
dc.date.issued 2026 -
dc.identifier.uri https://scholar.dgist.ac.kr/handle/20.500.11750/60762 -
dc.identifier.uri http://dgist.dcollection.net/common/orgView/200001017888 -
dc.description adult hippocampal neural stem cells, apoptosis, apoptotic protease activating factor 1, autophagic cell death, caspase-9, corticosterone -
dc.description.tableofcontents List of Contents
Abstract i
List of contents ii
List of figures iiii

Ⅰ. Introduction 1
II. Materials and Methods
2.1 Reagents and antibodies 6
2.2 Cell culture 6
2.3 Transfection and expression vector 6
2.4 Confocal imaging and analysis 6
2.5 Immunocytochemistry 7
2.6 Cell death assay 7
2.7 CASP9 activity assay 7
2.8 Western blotting analysis 7
2.9 Live cell imaging 8
2.10 Statistical analysis 8
III. Results
3.1 APAF1 depletion suppresses activation of apoptotic molecules following STS treatment 9
3.2 APAF1 is required for CORT-induced autophagic flux and ACD in ahNSC 11
3.3 CORT-induced non-apoptotic CASP9 activation depends on APAF1 in ahNSCs 13
3.4 CORT induces a slower generation of mitoROS than STS in ahNSCs 15
3.5 CORT treatment does not induce mitochondrial depolarization in ahNSCs 17
3.6 CORT induces less release of cytochrome c than STS in ahNSCs 19
3.7 APAF1 depletion alters ULK1 phosphorylation without inhibiting mTOR–AMPK–ULK1–ATG13 signaling in CORT-treated ahNSCs 21
3.8 APAF1 depletion selectively reduces ATG3 levels in CORT-treated ahNSC 24
ⅠV. Discussion 26
V. References 30
Abstract in Korean 33		 -
dc.format.extent 33 -
dc.language eng -
dc.publisher DGIST -
dc.title APAF1 is required for corticosterone-induced autophagic death of adult hippocampal neural stem cells -
dc.title.alternative APAF1의 코티코스테론 유도 성체 해마 신경줄기세포 자가포식 세포사멸에서의 역할 -
dc.type Thesis -
dc.identifier.doi 10.22677/THESIS.200001017888 -
dc.description.degree Master -
dc.contributor.department Department of Brain Sciences -
dc.contributor.coadvisor Byung-Hoon Lee -
dc.date.awarded 2026-08-01 -
dc.publisher.location Daegu -
dc.description.database dCollection -
dc.citation XT.BM 구64 202608 -
dc.date.accepted 2026-07-21 -
dc.contributor.alternativeDepartment 뇌과학과 -
dc.subject.keyword adult hippocampal neural stem cells, apoptosis, apoptotic protease activating factor 1, autophagic cell death, caspase-9, corticosterone -
dc.contributor.affiliatedAuthor Yeongwon Gu -
dc.contributor.affiliatedAuthor Seong-Woon Yu -
dc.contributor.affiliatedAuthor Byung-Hoon Lee -
dc.contributor.alternativeName 구영원 -
dc.contributor.alternativeName Seong-Woon Yu -
dc.contributor.alternativeName 이병훈 -
dc.rights.embargoReleaseDate 2031-08-31 -
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