Title

APAF1 is required for corticosterone-induced autophagic death of adult hippocampal neural stem cells

Alternative Title

APAF1의 코티코스테론 유도 성체 해마 신경줄기세포 자가포식 세포사멸에서의 역할

DGIST Authors

Yeongwon Gu ; Seong-Woon Yu ; Byung-Hoon Lee

Advisor

유성운

Co-Advisor(s)

Byung-Hoon Lee

Issued Date

2026

Awarded Date

2026-08-01

Type

Thesis

Description

adult hippocampal neural stem cells, apoptosis, apoptotic protease activating factor 1, autophagic cell death, caspase-9, corticosterone

Table Of Contents

List of Contents
Abstract i
List of contents ii
List of figures iiii

Ⅰ. Introduction 1
II. Materials and Methods
2.1 Reagents and antibodies 6
2.2 Cell culture 6
2.3 Transfection and expression vector 6
2.4 Confocal imaging and analysis 6
2.5 Immunocytochemistry 7
2.6 Cell death assay 7
2.7 CASP9 activity assay 7
2.8 Western blotting analysis 7
2.9 Live cell imaging 8
2.10 Statistical analysis 8
III. Results
3.1 APAF1 depletion suppresses activation of apoptotic molecules following STS treatment 9
3.2 APAF1 is required for CORT-induced autophagic flux and ACD in ahNSC 11
3.3 CORT-induced non-apoptotic CASP9 activation depends on APAF1 in ahNSCs 13
3.4 CORT induces a slower generation of mitoROS than STS in ahNSCs 15
3.5 CORT treatment does not induce mitochondrial depolarization in ahNSCs 17
3.6 CORT induces less release of cytochrome c than STS in ahNSCs 19
3.7 APAF1 depletion alters ULK1 phosphorylation without inhibiting mTOR–AMPK–ULK1–ATG13 signaling in CORT-treated ahNSCs 21
3.8 APAF1 depletion selectively reduces ATG3 levels in CORT-treated ahNSC 24
ⅠV. Discussion 26
V. References 30
Abstract in Korean 33

URI

https://scholar.dgist.ac.kr/handle/20.500.11750/60762
http://dgist.dcollection.net/common/orgView/200001017888

DOI

10.22677/THESIS.200001017888

Degree

Master

Department

Department of Brain Sciences

Publisher

DGIST

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