Title

Characterization of the Interaction between Human Protamine 1 and DNA

Alternative Title

인간 프로타민 1과 디옥시리보핵산 상호작용 특성 분석

DGIST Authors

Seongmin Kim ; Yoori Kim ; Chan Chung

Advisor

김유리

Co-Advisor(s)

Chan Chung

Issued Date

2026

Awarded Date

2026-08-01

Type

Thesis

Description

protamine, sperm, spermiogenesis, DNA interaction

Table Of Contents

I. Introduction 1
II. Materials and Methods 6
2.1 hP1 expression plasmid 6
2.2 Protein expression and purification of hP1 6
2.3 Western blot analysis 7
2.4 Preparation of DNA 8
2.5 Electrophoretic mobility shift assay （EMSA） 8
2.6 Preparation of single-molecule DNA substrates 9
2.7 Single-molecule imaging assay 9
2.8 Analysis of kymograph 11
III. Results 13
3.1 Expression and purification of hP1 13
3.2 Effect of DNA GC content on hP1 binding affinity 18
3.3 Estimation of DNA length bound by a single hP1 molecule 20
3.4 DNA condensation depending on hP1 concentration 22
IV. Discussion 26
References 30
Summary in Korean 35

URI

https://scholar.dgist.ac.kr/handle/20.500.11750/60774
http://dgist.dcollection.net/common/orgView/200001018031

DOI

10.22677/THESIS.200001018031

Degree

Master

Department

Department of New Biology

Publisher

DGIST

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