Cited 7 time in webofscience Cited 8 time in scopus

Single-Molecule Rotation for EGFR Conformational Dynamics in Live Cells

Title
Single-Molecule Rotation for EGFR Conformational Dynamics in Live Cells
Authors
Park, YoungchanShin, SangwonJin, HyeonggyuPark, JiseongHong, YeonkiChoi, JaeminJung, ByunghyuckSong, HyunjoonSeo, Dae Ha
DGIST Authors
Jung, ByunghyuckSeo, Dae Ha
Issue Date
2018-11
Citation
Journal of the American Chemical Society, 140(45), 15161-15165
Type
Article
Article Type
Article
Keywords
ENERGY-TRANSFERDIMERIZATIONACTIVATIONMEMBRANEMOBILITYMOTIONDOMAINGOLD
ISSN
0002-7863
Abstract
Monitoring the dynamics of proteins in live cells on appropriate spatiotemporal scales may provide key information regarding long-standing questions in molecular and cellular regulatory mechanisms. However, tools capable of imaging the conformational changes over time have been elusive. Here, we present a single-molecule stroboscopic imaging probes by developing gyroscopic plasmonic nanoparticles, allowing for replication of protein-protein interactions and the conformational dynamics based on rotational and lateral velocities. This study fundamentally monitors the rotational motion of a membrane protein, epidermal growth factor receptor (EGFR), to decipher undiscovered structural dynamics in live cells without any molecular perturbations. This method offers a strategy to visualize assemblies and conformational changes, and provides unique insights into the mechanism underlying the molecular dynamics for receptors. © 2018 American Chemical Society.
URI
http://hdl.handle.net/20.500.11750/9449
DOI
10.1021/jacs.8b09037
Publisher
American Chemical Society
Related Researcher
  • Author Jung, Byunghyuck Asymmetric Organic Synthesis and Drug Synthesis Laboratory
  • Research Interests Organic Synthesis; Organo-transition metal chemistry; Catalytic Asymmetric Synthesis; Synthetic Methodologies; Synthesis of Natural Products and Drugs
Files:
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Collection:
Department of Emerging Materials ScienceSMALL LAB(Single Molecule Approaches to ceLL Lab)1. Journal Articles
Department of Emerging Materials ScienceAsymmetric Organic Synthesis and Drug Synthesis Laboratory1. Journal Articles


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