Cited 7 time in
Cited 8 time in
Single-Molecule Rotation for EGFR Conformational Dynamics in Live Cells
- Single-Molecule Rotation for EGFR Conformational Dynamics in Live Cells
- Park, Youngchan; Shin, Sangwon; Jin, Hyeonggyu; Park, Jiseong; Hong, Yeonki; Choi, Jaemin; Jung, Byunghyuck; Song, Hyunjoon; Seo, Dae Ha
- DGIST Authors
- Jung, Byunghyuck; Seo, Dae Ha
- Issue Date
- Journal of the American Chemical Society, 140(45), 15161-15165
- Article Type
- ENERGY-TRANSFER; DIMERIZATION; ACTIVATION; MEMBRANE; MOBILITY; MOTION; DOMAIN; GOLD
- Monitoring the dynamics of proteins in live cells on appropriate spatiotemporal scales may provide key information regarding long-standing questions in molecular and cellular regulatory mechanisms. However, tools capable of imaging the conformational changes over time have been elusive. Here, we present a single-molecule stroboscopic imaging probes by developing gyroscopic plasmonic nanoparticles, allowing for replication of protein-protein interactions and the conformational dynamics based on rotational and lateral velocities. This study fundamentally monitors the rotational motion of a membrane protein, epidermal growth factor receptor (EGFR), to decipher undiscovered structural dynamics in live cells without any molecular perturbations. This method offers a strategy to visualize assemblies and conformational changes, and provides unique insights into the mechanism underlying the molecular dynamics for receptors. © 2018 American Chemical Society.
- American Chemical Society
- Related Researcher
Asymmetric Organic Synthesis and Drug Synthesis Laboratory
Organic Synthesis; Organo-transition metal chemistry; Catalytic Asymmetric Synthesis; Synthetic Methodologies; Synthesis of Natural Products and Drugs
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- Department of Emerging Materials ScienceSMALL LAB(Single Molecule Approaches to ceLL Lab)1. Journal Articles
Department of Emerging Materials ScienceAsymmetric Organic Synthesis and Drug Synthesis Laboratory1. Journal Articles
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