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Comparison of Lipid Profiles in Head and Brain Samples of Drosophila Melanogaster Using Electrospray Ionization Mass Spectrometry (ESI-MS)

Title
Comparison of Lipid Profiles in Head and Brain Samples of Drosophila Melanogaster Using Electrospray Ionization Mass Spectrometry (ESI-MS)
Authors
Jang, Hyun JunPark, Jeong HyangLee, Ga SeulLee, Sung-BaeMoon, Jeong HeeChoi, Joon SigLee, Tae GeolYoon, Sohee
DGIST Authors
Jang, Hyun Jun; Park, Jeong Hyang; Lee, Ga Seul; Lee, Sung-Bae; Moon, Jeong Hee; Choi, Joon Sig; Lee, Tae Geol; Yoon, Sohee
Issue Date
2019-03
Citation
Mass Spectrometry Letters, 10(1), 11-17
Type
Article
Article Type
Article
Author Keywords
Lipid profileDrosophila samplingDrosophila brainmass spectrometry (MS)electrospray ionization (ESI)
Keywords
OF-FLIGHT INSTRUMENTMODELCOMPLEXESDRUGS
ISSN
2233-4203
Abstract
Drosophila melanogaster (fruits fly) is a representative model system widely used in biological studies because its brain function and basic cellular processes are similar to human beings. The whole head of the fly is often used to obtain the key function in brain-related diseases like degenerative brain diseases; however the biomolecular distribution of the head may be slightly different from that of a brain. Herein, lipid profiles of the head and dissected brain samples of Drosophila were studied using electrospray ionization-mass spectrometry (ESI-MS). According to the sample types, the detection of phospholipid ions was suppressed by triacylglycerol (TAG), or the specific phospholipid signals that are absent in the mass spectrum were measured. The lipid distribution was found to be different in the wild-type and the microRNA-14 deficiency model (miR-14Δ1) with abnormal lipid metabolism. A few phospholipids were also profiled by comparison of the head and the brain in two fly model systems. The mass spectra showed that the phospholipid distributions in the miR-14Δ1 model and the wild-type were different, and principal component analysis revealed a correlation between some phospholipids (phosphatidylethanolamine (PE), phosphatidylinositol (PI), and phosphatidylserine (PS)) in miR-14Δ1. The overall results suggested that brain-related lipids should be profiled using fly samples after dissection for more accurate analysis. © Korean Society for Mass Spectrometry.
URI
http://hdl.handle.net/20.500.11750/9775
DOI
10.5478/MSL.2019.10.1.11
Publisher
Korean Society for Mass Spectrometry
Related Researcher
  • Author Lee, Sung Bae Laboratory of Neurodegenerative Diseases and Aging
  • Research Interests Cellular mechanism of neurodegenerative diseases; Neuronal maintenance and remodeling; 퇴행성 뇌질환의 세포기전; 신경계 유지 및 리모델링 연구
Files:
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Collection:
Department of Brain SciencesLaboratory of Neurodegenerative Diseases and Aging1. Journal Articles


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