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Acoustic Trapping Technique for Studying Calcium Response of a Suspended Breast Cancer Cell: Determination of its Invasion Potentials
- Acoustic Trapping Technique for Studying Calcium Response of a Suspended Breast Cancer Cell: Determination of its Invasion Potentials
- Youn, Sangyeon; Choi, Jin Woo; Lee, Jun Su; Kim, Jihun; Yang, In-Hwan; Chang, Jin Ho; Kim, Hee Chan; Hwang, Jae Youn
- DGIST Authors
- Chang, Jin Ho; Hwang, Jae Youn
- Issue Date
- IEEE Transactions on Ultrasonics, Ferroelectrics, and Frequency Control, 66(4), 737-746
- Article Type
- Author Keywords
- Breast cancer cell; calcium fluorescence imaging; high-frequency ultrasound; invasion potential; noncontact single-beam acoustic trapping
- ULTRASOUND MICROBEAM STIMULATION; HIGH-FREQUENCY; MECHANOTRANSDUCTION; DEFORMATION; TRANSDUCERS; FORCE
- A noncontact single-beam acoustic trapping technique has proven to be a promising tool for cell manipulation and characterization that provide essential knowledge for a variety of biomedical applications. Here, we investigated cell characteristics as to whether the calcium responses of suspended breast cancer cells to different acoustic trapping forces are related to their invasiveness. For this, we combined a single-beam acoustic trapping system with a 30-MHz press-focused lithium niobate ultrasound transducer and an epifluorescence microscope. Using the system, intracellular calcium changes of suspended MDA-MB-231 (highly invasive) and MCF-7 (weakly invasive) cells were monitored while trapping the cells at different acoustic pressures. The results showed that a single suspended breast cancer cell isolated by the acoustic microbeam behaved differently on the calcium elevation in response to changes in acoustic trapping force, depending on its invasiveness. In particular, the MDA-MB-231 cells exhibited higher calcium elevation than MCF-7 cells when each cell was trapped at low acoustic pressure. Based on these results, we believe that the single-beam acoustic trapping technique has high potential as an alternative tool for determining the degree of invasiveness of suspended breast cancer cells.
- Institute of Electrical and Electronics Engineers
- Related Researcher
Hwang, Jae Youn
MBIS(Multimodal Biomedical Imaging and System) Laboratory
Multimodal Imaging; High-Frequency Ultrasound Microbeam; Ultrasound Imaging and Analysis; 스마트 헬스케어; Biomedical optical system
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- Department of Information and Communication EngineeringMedical Acoustic Fusion Innovation Lab.1. Journal Articles
Department of Information and Communication EngineeringMBIS(Multimodal Biomedical Imaging and System) Laboratory1. Journal Articles
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