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Acoustic Trapping Technique for Studying Calcium Response of a Suspended Breast Cancer Cell: Determination of its Invasion Potentials

Title
Acoustic Trapping Technique for Studying Calcium Response of a Suspended Breast Cancer Cell: Determination of its Invasion Potentials
Authors
Youn, SangyeonChoi, Jin WooLee, Jun SuKim, JihunYang, In-HwanChang, Jin HoKim, Hee ChanHwang, Jae Youn
DGIST Authors
Chang, Jin HoHwang, Jae Youn
Issue Date
2019-04
Citation
IEEE Transactions on Ultrasonics, Ferroelectrics, and Frequency Control, 66(4), 737-746
Type
Article
Article Type
Article
Author Keywords
Breast cancer cellcalcium fluorescence imaginghigh-frequency ultrasoundinvasion potentialnoncontact single-beam acoustic trapping
Keywords
ULTRASOUND MICROBEAM STIMULATIONHIGH-FREQUENCYMECHANOTRANSDUCTIONDEFORMATIONTRANSDUCERSFORCE
ISSN
0885-3010
Abstract
A noncontact single-beam acoustic trapping technique has proven to be a promising tool for cell manipulation and characterization that provide essential knowledge for a variety of biomedical applications. Here, we investigated cell characteristics as to whether the calcium responses of suspended breast cancer cells to different acoustic trapping forces are related to their invasiveness. For this, we combined a single-beam acoustic trapping system with a 30-MHz press-focused lithium niobate ultrasound transducer and an epifluorescence microscope. Using the system, intracellular calcium changes of suspended MDA-MB-231 (highly invasive) and MCF-7 (weakly invasive) cells were monitored while trapping the cells at different acoustic pressures. The results showed that a single suspended breast cancer cell isolated by the acoustic microbeam behaved differently on the calcium elevation in response to changes in acoustic trapping force, depending on its invasiveness. In particular, the MDA-MB-231 cells exhibited higher calcium elevation than MCF-7 cells when each cell was trapped at low acoustic pressure. Based on these results, we believe that the single-beam acoustic trapping technique has high potential as an alternative tool for determining the degree of invasiveness of suspended breast cancer cells.
URI
http://hdl.handle.net/20.500.11750/9777
DOI
10.1109/tuffc.2019.2894662
Publisher
Institute of Electrical and Electronics Engineers
Related Researcher
  • Author Hwang, Jae Youn MBIS(Multimodal Biomedical Imaging and System) Laboratory
  • Research Interests Multimodal Imaging; High-Frequency Ultrasound Microbeam; Ultrasound Imaging and Analysis; 스마트 헬스케어; Biomedical optical system
Files:
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Collection:
Department of Information and Communication EngineeringMedical Acoustic Fusion Innovation Lab.1. Journal Articles
Department of Information and Communication EngineeringMBIS(Multimodal Biomedical Imaging and System) Laboratory1. Journal Articles


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