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Graphene-Coated Glass Substrate for Continuous Wave Laser Desorption and Atmospheric Pressure Mass Spectrometric Imaging of a Live Hippocampal Tissue

Title
Graphene-Coated Glass Substrate for Continuous Wave Laser Desorption and Atmospheric Pressure Mass Spectrometric Imaging of a Live Hippocampal Tissue
Authors
Kim, Jae YoungLim, HeejinLee, Sun YoungSong, CheolPark, Ji-WonShin, Hyeon HoLim, Dong-KwonMoon, Dae Won
DGIST Authors
Kim, Jae Young; Song, CheolMoon, Dae Won
Issue Date
2019-07
Citation
ACS Applied Materials and Interfaces, 11(30), 27153-27161
Type
Article
Article Type
Article
Author Keywords
mass spectrometry imaginggraphene-coated substratelaser desorptionambient mass spectrometryhippocampal tissue
Keywords
REAL-TIMEAMBIENT IONIZATIONSMALL MOLECULESDESORPTION/IONIZATIONMETABOLITESLAYERMS
ISSN
1944-8244
Abstract
The atmospheric pressure mass spectrometric (AP-MS) imaging technology combined with an inverted optical microscopic system is a powerful tool for determining the presence and spatial distributions of specific biomolecules of interest in live tissues. Efficient desorption and ionization are essential to acquire mass spectrometric (MS) information in an ambient environment. In this study, we demonstrate a new and efficient desorption process using a graphene-coated glass substrate and a continuous wave (CW) laser for high-resolution AP-MS imaging of a live hippocampal tissue. We found that desorption of biomolecules in a live tissue slice was possible with the aid of a graphene-coated glass substrate and indirect application of a 532 nm CW laser on the graphene substrate. Interestingly, the desorption efficiency of a live tissue on the graphene-coated substrate was strongly dependent on the number of graphene layers. Single-layer graphene was found to be the most sensitive substrate for efficient desorption and reproducible high-resolution hippocampal tissue imaging applications. The subsequent ionization process using nonthermal plasma generated sufficient amounts of molecular ions to obtain high-resolution two-dimensional MS images of the cornu ammonis and the dentate gyrus regions of the hippocampus. Therefore, graphene-coated substrates could be a promising platform to induce an efficient desorption process essential for highly reproducible ambient MS imaging. Copyright © 2019 American Chemical Society.
URI
http://hdl.handle.net/20.500.11750/10486
DOI
10.1021/acsami.9b02620
Publisher
American Chemical Society
Related Researcher
  • Author Moon, Dae Won NanoBio Imaging Laboratory
  • Research Interests Coherent Raman Scattering; Surface Plasmon Resonance Imaging Ellipsometry; Imaging Mass Spectrometry; Time-of-flight Medium Energy Ion Scattering
Files:
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Collection:
Department of Robotics EngineeringRobotics Engineering Research Center1. Journal Articles
Department of Robotics EngineeringIBOM(Intelligent Bio-Optomechatronics) Lab1. Journal Articles
Department of New BiologyNanoBio Imaging Laboratory1. Journal Articles


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