Cited 10 time in webofscience Cited 11 time in scopus

Tanycytic TSPO Inhibition Induces Lipophagy to Regulate Lipid Metabolism and Improve Energy Balance

Title
Tanycytic TSPO Inhibition Induces Lipophagy to Regulate Lipid Metabolism and Improve Energy Balance
Authors
Kim, SeolsongKim, NayounPark, SeokjaeJeon, YoonjeongLee, JaemeunYoo, Seung JunLee, Ji-WonMoon, CheilYu, Seong-WoonKim, Eun-Kyoung
DGIST Authors
Moon, CheilYu, Seong-WoonKim, Eun-Kyoung
Issue Date
2020-07
Citation
Autophagy, 16(7), 1200-1220
Type
Article
Article Type
Article
Author Keywords
AMPKautophagyenergy balanceenergy expenditurefood intakehypothalamuslipid metabolismlipophagytanycyteTSPO
Keywords
TRIGGERS CALCIUM MOBILIZATIONBENZODIAZEPINE-RECEPTOR PBRTRANSLOCATOR-PROTEIN TSPOKINASE KINASE-BETA18 KDA LIGANDHYPOTHALAMIC TANYCYTESFOOD-INTAKEEMERGING ROLESINGLE-CELLAUTOPHAGY
ISSN
1554-8627
Abstract
Hypothalamic glial cells named tanycytes, which line the 3rd ventricle (3V), are components of the hypothalamic network that regulates a diverse array of metabolic functions for energy homeostasis. Herein, we report that TSPO (translocator protein), an outer mitochondrial protein, is highly enriched in tanycytes and regulates homeostatic responses to nutrient excess as a potential target for an effective intervention in obesity. Administration of a TSPO ligand, PK11195, into the 3V, and tanycyte-specific deletion of Tspo reduced food intake and elevated energy expenditure, leading to negative energy balance in a high-fat diet challenge. Ablation of tanycytic Tspo elicited AMPK-dependent lipophagy, breaking down lipid droplets into free fatty acids, thereby elevating ATP in a lipid stimulus. Our findings suggest that tanycytic TSPO affects systemic energy balance through macroautophagy/autophagy-regulated lipid metabolism, and highlight the physiological significance of TSPO in hypothalamic lipid sensing and bioenergetics in response to overnutrition. Abbreviations: 3V: 3rd ventricle; ACAC: acetyl-Coenzyme A carboxylase; AGRP: agouti related neuropeptide; AIF1/IBA1: allograft inflammatory factor 1; AMPK: AMP-activated protein kinase; ARC: arcuate nucleus; Atg: autophagy related; Bafilo: bafilomycin A1; CAMKK2: calcium/calmodulin-dependent protein kinase kinase 2, beta; CCCP: carbonyl cyanide m-chlorophenylhydrazone; CNS: central nervous system; COX4I1: cytochrome c oxidase subunit 4I1; FFA: free fatty acid; GFAP: glial fibrillary acidic protein; HFD: high-fat diet; ICV: intracerebroventricular; LAMP2: lysosomal-associated membrane protein 2; LD: lipid droplet; MAP1LC3B/LC3B: microtubule-associated protein 1 light chain 3 beta; MBH: mediobasal hypothalamus; ME: median eminence; MEF: mouse embryonic fibroblast; NCD: normal chow diet; NEFM/NFM: neurofilament medium; NPY: neuropeptide Y; OL: oleic acid; POMC: pro-opiomelanocortin-alpha; PRKN/Parkin: parkin RBR E3 ubiquitin protein ligase; Rax: retina and anterior neural fold homeobox; RBFOX3/NeuN: RNA binding protein, fox-1 homolog (C. elegans) 3; RER: respiratory exchange ratio; siRNA: small interfering RNA; SQSTM1: sequestosome 1; TG: triglyceride; TSPO: translocator protein; ULK1: unc-51 like kinase 1; VCO2: carbon dioxide production; VMH: ventromedial hypothalamus; VO2: oxygen consumption. © 2019, © 2019 The Author(s). Published by Informa UK Limited, trading as Taylor & Francis Group.
URI
http://hdl.handle.net/20.500.11750/10613
DOI
10.1080/15548627.2019.1659616
Publisher
Taylor and Francis Inc.
Related Researcher
  • Author Moon, Cheil Laboratory of Chemical Senses
  • Research Interests Brain convergent science based on chemical senses; olfaction; 감각신경계 기반 뇌융합과학; 후각 신경계
Files:
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Collection:
Department of Brain and Cognitive SciencesLaboratory of Chemical Senses1. Journal Articles
Department of Brain and Cognitive SciencesLaboratory of Neuronal Cell Death1. Journal Articles
Department of Brain and Cognitive SciencesLab of Neuro-Metabolism & Neurometabolomic Research Center1. Journal Articles


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