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Methionine synthase 1 provides methionine for activation of the GLR3.5 Ca2+ channel and regulation of germination in Arabidopsis

Title
Methionine synthase 1 provides methionine for activation of the GLR3.5 Ca2+ channel and regulation of germination in Arabidopsis
Authors
Ju, ChuanliKong, DongdongLee, YureeGe, GegeSong, YananLiu, JiawenKwak, June Myoung
DGIST Authors
Kwak, June Myoung
Issue Date
2020-01
Citation
Journal of Experimental Botany, 71(1), 178-187
Type
Article
Article Type
Article
Author Keywords
Calcium channelsAtGLR3.5methioninemethionine synthase 1seed germination
Keywords
GLUTAMATE-RECEPTORSEED-GERMINATIONMESSENGER-RNAABSCISIC-ACIDION CHANNELSGENEBIOSYNTHESISROLESMATURATIONDORMANCY
ISSN
0022-0957
Abstract
Seed germination is a developmental process regulated by numerous internal and external cues. Our previous studies have shown that calcium influx mediated by the Arabidopsis glutamate receptor homolog 3.5 (AtGLR3.5) modulates the expression of the ABSCISIC ACID INSENSITIVE 4 (ABI4) transcription factor during germination and that L-methionine (L-Met) activates AtGLR3.1/3.5 Ca2+ channels in guard cells. However, it is not known whether L-Met participates in regulation of germination and what cellular mechanism is responsible for Met production during germination. Here, we describe Arabidopsis methionine synthase 1 (AtMS1), which acts in the final step of Met biosynthesis, synthesizes the Met required for the activation of AtGLR3.5 Ca2+ channels whose expression is up-regulated during germination, leading to the regulation of seed germination. We show that exogenous L-Met promotes germination in an AtGRL3.5-dependent manner. We also demonstrate that L-Met directly regulates the AtGLR3.5-mediated increase in cytosolic Ca2+ level in seedlings. We provide pharmacological and genetic evidence that Met synthesized via AtMS1 acts upstream of the AtGLR3.5-mediated Ca2+ signal and regulates the expression of ABI4, a major regulator in the abscisic acid response in seeds. Overall, our results link AtMS1, L-Met, the AtGLR3.5 Ca2+ channel, Ca2+ signals, and ABI4, and shed light on the physiological role and molecular mechanism of L-Met in germination. © The Author(s) 2019. Published by Oxford University Press on behalf of the Society for Experimental Biology.
URI
http://hdl.handle.net/20.500.11750/11430
DOI
10.1093/jxb/erz431
Publisher
Oxford University Press
Related Researcher
  • Author Kwak, June Myoung Lab of Cell Signaling and Development
  • Research Interests Calcium Signaling; ABA;ROS Signaling; plant development; Development of Abscission; Culluar Features; Signaling network; Environmental Regulation
Files:
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Collection:
Department of New BiologyLab of Cell Signaling and Development1. Journal Articles


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