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Targeted removal of leukemia cells from the circulating system by whole-body magnetic hyperthermia in mice

Title
Targeted removal of leukemia cells from the circulating system by whole-body magnetic hyperthermia in mice
Authors
Faruque, Md Hasan AlChoi, Eun-SookLee, Hyo-RyongKim, Jung-HeePark, SukhoKim, Eunjoo
DGIST Authors
Kim, Jung-Hee; Park, SukhoKim, Eunjoo
Issue Date
2020-01
Citation
Nanoscale, 12(4), 2773-2786
Type
Article
Article Type
Article
Keywords
IRON-OXIDE NANOPARTICLESADHESION MOLECULECANCERDELIVERYBLOODCHEMOTHERAPY
ISSN
2040-3364
Abstract
Until now, magnetic hyperthermia was used to remove solid tumors by targeting magnetic nanoparticles (MNPs) to tumor sites. In this study, leukemia cells in the bloodstream were directly removed by whole-body hyperthermia, using leukemia cell-specific MNPs. An epithelial cellular adhesion molecule (EpCAM) antibody was immobilized on the surface of MNPs (EpCAM-MNPs) to introduce the specificity of MNPs to leukemia cells. The viability of THP1 cells (human monocytic leukemia cells) was decreased to 40.8% of that in control samples by hyperthermia using EpCAM-MNPs. In AKR mice, an animal model of lymphoblastic leukemia, the number of leukemia cells was measured following the intravenous injection of EpCAM-MNPs and subsequent whole-body hyperthermia treatment. The result showed that the leukemia cell number was also decreased to 43.8% of that without the treatment of hyperthermia, determined by Leishman staining of leukemia cells. To support the results, simulation analysis of heat transfer from MNPs to leukemia cells was performed using COMSOL Multiphysics simulation software. The surface temperature of leukemia cells adhered to EpCAM-MNPs was predicted to be increased to 82 °C, whereas the temperature of free cells without adhered MNPs was predicted to be 38 °C. Taken together, leukemia cells were selectively removed by magnetic hyperthermia from the bloodstream, because EpCAM-modified magnetic particles were specifically attached to leukemia cell surfaces. This approach has the potential to remove metastatic cancer cells, and pathogenic bacteria and viruses floating in the bloodstream. © 2020 The Royal Society of Chemistry.
URI
http://hdl.handle.net/20.500.11750/11574
DOI
10.1039/c9nr06730b
Publisher
Royal Society of Chemistry
Related Researcher
  • Author Kim, Eunjoo  
  • Research Interests Biomarker, liquid biopsy, molecular diagnosis, nanobiosensor, drug delivery, exosome
Files:
There are no files associated with this item.
Collection:
Division of Electronics & Information System1. Journal Articles
Department of Robotics EngineeringMultiscale Biomedical Robotics Laboratory1. Journal Articles


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