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Multiplex Protein Imaging with Secondary Ion Mass Spectrometry Using Metal Oxide Nanoparticle-Conjugated Antibodies
- Multiplex Protein Imaging with Secondary Ion Mass Spectrometry Using Metal Oxide Nanoparticle-Conjugated Antibodies
- Moon, Dae Won; Park, Young Ho; Lee, Sun Young; Lim, Heejin; Kwak, SuHwa; Kim, Minseok S.; Kim, Hyunmin; Kim, Eunjoo; Jung, Yebin; Hoe, Hyang-Sook; Kim, Sungjee; Lim, Dong-Kwon; Kim, Chul-Hoon; In, Su-Il
- DGIST Authors
- Moon, Dae Won; Kim, Minseok S.; Kim, Hyunmin; Kim, Eunjoo; In, Su-Il
- Issue Date
- ACS Applied Materials and Interfaces, 12(15), 18056-18064
- Article Type
- Author Keywords
- secondary ion mass spectrometry; multiplex protein imaging; metal oxide nanoparticle; Alzheimer' s disease; protein cluster proximity analysis
- CELL; RESOLUTION; MICROSCOPY; STABILITY; LIPIDS; MODEL
- In spite of recent developments in mass spectrometry imaging techniques, high-resolution multiplex protein bioimaging techniques are required to unveil the complex inter- and intracellular biomolecular interactions for accurate understanding of life phenomena and disease mechanisms. Herein, we report multiplex protein imaging with secondary ion mass spectrometry (SIMS) using metal oxide nanoparticle (MONP)-conjugated antibodies with <300 nm spatial resolution in the low ion dose without ion beam damage because of the high secondary ion yields of the MONPs, which can provide simultaneous imaging of several proteins, especially from cell membranes. We applied our new imaging technique for the study of hippocampal tissue samples from control and Alzheimer's disease (AD) model mice; the proximity of protein clusters in the hippocampus CA1 region showed intriguing dependence on aging and AD progress, suggesting that protein cluster proximity may be helpful for understanding pathological pathways in the microscopic cellular level. © 2020 American Chemical Society.
- American Chemical Society
- Related Researcher
Biomarker, liquid biopsy, molecular diagnosis, nanobiosensor, drug delivery, exosome
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