Cited 4 time in webofscience Cited 3 time in scopus

Multiplex Protein Imaging with Secondary Ion Mass Spectrometry Using Metal Oxide Nanoparticle-Conjugated Antibodies

Title
Multiplex Protein Imaging with Secondary Ion Mass Spectrometry Using Metal Oxide Nanoparticle-Conjugated Antibodies
Authors
Moon, Dae WonPark, Young HoLee, Sun YoungLim, HeejinKwak, SuHwaKim, Minseok S.Kim, HyunminKim, EunjooJung, YebinHoe, Hyang-SookKim, SungjeeLim, Dong-KwonKim, Chul-HoonIn, Su-Il
DGIST Authors
Moon, Dae WonKim, Minseok S.Kim, HyunminKim, EunjooIn, Su-Il
Issue Date
2020-04
Citation
ACS Applied Materials and Interfaces, 12(15), 18056-18064
Type
Article
Article Type
Article
Author Keywords
secondary ion mass spectrometrymultiplex protein imagingmetal oxide nanoparticleAlzheimer&aposs diseaseprotein cluster proximity analysis
Keywords
CELLRESOLUTIONMICROSCOPYSTABILITYLIPIDSMODEL
ISSN
1944-8244
Abstract
In spite of recent developments in mass spectrometry imaging techniques, high-resolution multiplex protein bioimaging techniques are required to unveil the complex inter- and intracellular biomolecular interactions for accurate understanding of life phenomena and disease mechanisms. Herein, we report multiplex protein imaging with secondary ion mass spectrometry (SIMS) using metal oxide nanoparticle (MONP)-conjugated antibodies with <300 nm spatial resolution in the low ion dose without ion beam damage because of the high secondary ion yields of the MONPs, which can provide simultaneous imaging of several proteins, especially from cell membranes. We applied our new imaging technique for the study of hippocampal tissue samples from control and Alzheimer's disease (AD) model mice; the proximity of protein clusters in the hippocampus CA1 region showed intriguing dependence on aging and AD progress, suggesting that protein cluster proximity may be helpful for understanding pathological pathways in the microscopic cellular level. © 2020 American Chemical Society.
URI
http://hdl.handle.net/20.500.11750/11783
DOI
10.1021/acsami.9b21800
Publisher
American Chemical Society
Related Researcher
  • Author Kim, Eunjoo  
  • Research Interests Biomarker, liquid biopsy, molecular diagnosis, nanobiosensor, drug delivery, exosome
Files:
There are no files associated with this item.
Collection:
Department of New BiologyBioDr. Lab - Nanobiomedicine1. Journal Articles
Department of New BiologyNanoBio Imaging Laboratory1. Journal Articles
Division of Biotechnology1. Journal Articles
Division of Electronics & Information System1. Journal Articles
Department of Energy Science and EngineeringGreen and Renewable Energy for Endless Nature(GREEN) Lab1. Journal Articles


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