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Cathepsin A regulates pluripotency, proliferation and differentiation in mouse embryonic stem cells

Title
Cathepsin A regulates pluripotency, proliferation and differentiation in mouse embryonic stem cells
Authors
Park, SongHuang, HaiKwon, WookbongKim, Hee‐YeonPark, Jin‐KyuHan, Jee EunCho, Gil‐JaeHan, Se‐HyeonSung, YonghunRyoo, Zae YoungKim, Myoung OkChoi, Seong-Kyoon
DGIST Authors
Kwon, Wookbong; Choi, Seong-Kyoon
Issue Date
2021-01
Citation
Cell Biochemistry and Function, 39(1), 67-76
Type
Article
Article Type
Article
Author Keywords
Cathepsin Acell cycledifferentiationpluripotencyproliferation
Keywords
DOWN-REGULATIONSELF-RENEWAL
ISSN
0263-6484
Abstract
Mouse embryonic stem cells (mESCs) are pluripotent cells that possess the ability to self-renew and differentiate into three germ layers. Owing to these characteristics, mESCs act as important models for stem cell research and are being used in many clinical applications. Among the many cathepsins, cathepsin A (Ctsa), a serine protease, affects the function and properties of stem cells. However, studies on the role of Ctsa in stem cells are limited. Here, we observed a significant increase in Ctsa expression during mESC differentiation at protein levels. Furthermore, we established Ctsa knockdown mESCs. Ctsa knockdown led to Erk1/2 phosphorylation, which in turn inhibited the pluripotency of mESCs and induced G2/M cell cycle arrest to inhibit mESC proliferation. The knockdown also induced abnormal differentiation in mESCs and aberrant expression of differentiation markers. Furthermore, we identified inhibition of teratoma formation in nude mice. Our results suggested that Ctsa affects mESC pluripotency, proliferation, cell cycle and differentiation, and highlighted the potential of Ctsa to act as a core factor that can regulate various mESC properties. Significance of the study: Our results indicate that cathepsin A (Ctsa) affects the properties of mESCs. Inhibition of Ctsa resulted in a decrease in the pluripotency of mouse embryonic stem cells (mESCs). Further, Ctsa suppression resulted in decreased proliferation via cell cycle arrest. Moreover, Ctsa inhibition reduced differentiation abilities and formation of teratoma in mESCs. Our results demonstrated that Ctsa is an important factor controlling mESC abilities. © 2020 John Wiley & Sons Ltd
URI
http://hdl.handle.net/20.500.11750/12067
DOI
10.1002/cbf.3554
Publisher
John Wiley and Sons Ltd
Related Researcher
Files:
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Collection:
Division of Biotechnology1. Journal Articles


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