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Protocol for Quantitative Analysis of Synaptic Vesicle Clustering in Axons of Cultured Neurons
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dc.contributor.author Um, Ji Won -
dc.contributor.author Han, Kyung Ah -
dc.contributor.author Choi, Se-Young -
dc.contributor.author Ko, Jaewon -
dc.date.accessioned 2021-01-22T06:42:21Z -
dc.date.available 2021-01-22T06:42:21Z -
dc.date.created 2020-08-24 -
dc.date.issued 2020-09 -
dc.identifier.issn 2666-1667 -
dc.identifier.uri http://hdl.handle.net/20.500.11750/12578 -
dc.description.abstract Clustering of synaptic vesicles along the neuronal axons is a critical mechanism underpinning proper synaptic transmission. Here, we provide a detailed protocol for analyzing the distribution of synaptic vesicles in presynaptic boutons of cultured neurons. The protocol covers preparation of cultured neurons, expression of synaptic vesicle-enriched proteins, and quantification procedures. Utilizing neurons from postnatal transgenic mice, this method can be applied to investigate the roles of synaptic genes in regulating vesicle dynamics at synaptic sites. For complete details on the use and execution of this protocol, please refer to Han et al. (2020a). © 2020 The Author(s) -
dc.language English -
dc.publisher Elsevier -
dc.title Protocol for Quantitative Analysis of Synaptic Vesicle Clustering in Axons of Cultured Neurons -
dc.type Article -
dc.identifier.doi 10.1016/j.xpro.2020.100095 -
dc.identifier.scopusid 2-s2.0-85094877397 -
dc.identifier.bibliographicCitation Um, Ji Won. (2020-09). Protocol for Quantitative Analysis of Synaptic Vesicle Clustering in Axons of Cultured Neurons. STAR Protocols, 1(2), 100095. doi: 10.1016/j.xpro.2020.100095 -
dc.description.isOpenAccess TRUE -
dc.citation.number 2 -
dc.citation.startPage 100095 -
dc.citation.title STAR Protocols -
dc.citation.volume 1 -
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엄지원
Um, Ji Won엄지원

Department of Brain Sciences

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