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dc.contributor.author Um, Ji Won -
dc.contributor.author Han, Kyung Ah -
dc.contributor.author Choi, Se-Young -
dc.contributor.author Ko, Jaewon -
dc.date.accessioned 2021-01-22T06:42:21Z -
dc.date.available 2021-01-22T06:42:21Z -
dc.date.created 2020-08-24 -
dc.date.created 2020-08-24 -
dc.date.created 2020-08-24 -
dc.date.issued 2020-09 -
dc.identifier.citation STAR Protocols, v.1, no.2, pp.100095 -
dc.identifier.issn 2666-1667 -
dc.identifier.uri http://hdl.handle.net/20.500.11750/12578 -
dc.description.abstract Clustering of synaptic vesicles along the neuronal axons is a critical mechanism underpinning proper synaptic transmission. Here, we provide a detailed protocol for analyzing the distribution of synaptic vesicles in presynaptic boutons of cultured neurons. The protocol covers preparation of cultured neurons, expression of synaptic vesicle-enriched proteins, and quantification procedures. Utilizing neurons from postnatal transgenic mice, this method can be applied to investigate the roles of synaptic genes in regulating vesicle dynamics at synaptic sites. For complete details on the use and execution of this protocol, please refer to Han et al. (2020a). © 2020 The Author(s) -
dc.language English -
dc.publisher Elsevier -
dc.title Protocol for Quantitative Analysis of Synaptic Vesicle Clustering in Axons of Cultured Neurons -
dc.type Article -
dc.identifier.doi 10.1016/j.xpro.2020.100095 -
dc.identifier.scopusid 2-s2.0-85094877397 -
dc.type.local Article(Overseas) -
dc.type.rims ART -
dc.description.journalClass 1 -
dc.citation.publicationname STAR Protocols -
dc.contributor.localauthor Um, Ji Won -
dc.contributor.localauthor Han, Kyung Ah -
dc.contributor.localauthor Choi, Se-Young -
dc.contributor.localauthor Ko, Jaewon -
dc.contributor.nonIdAuthor Han, Kyung Ah -
dc.contributor.nonIdAuthor Choi, Se-Young -
dc.identifier.citationVolume 1 -
dc.identifier.citationNumber 2 -
dc.identifier.citationStartPage 100095 -
dc.identifier.citationTitle STAR Protocols -
dc.type.journalArticle Protocol -
dc.description.isOpenAccess Y -

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