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dc.contributor.author Youn, Sangyeon -
dc.contributor.author Lee, Kyungsu -
dc.contributor.author Son, Jeehoon -
dc.contributor.author Yang, In-Hwan -
dc.contributor.author Hwang, Jae Youn -
dc.date.accessioned 2021-01-22T08:00:02Z -
dc.date.available 2021-01-22T08:00:02Z -
dc.date.created 2020-06-16 -
dc.date.created 2020-06-16 -
dc.date.created 2020-06-16 -
dc.date.issued 2020-06 -
dc.identifier.citation Biomedical Optics Express, v.11, no.6, pp.2976 - 2995 -
dc.identifier.issn 2156-7085 -
dc.identifier.uri http://hdl.handle.net/20.500.11750/12857 -
dc.description.abstract A single-beam acoustic trapping technique has been shown to be very useful for determining the invasiveness of suspended breast cancer cells in an acoustic trap with a manual calcium analysis method. However, for the rapid translation of the technology into the clinic, the development of an efficient/accurate analytical method is needed. We, therefore, develop a fully-automatic deep learning-based calcium image analysis algorithm for determining the invasiveness of suspended breast cancer cells using a single-beam acoustic trapping system. The algorithm allows to segment cells, find trapped cells, and quantify their calcium changes over time. For better segmentation of calcium fluorescent cells even with vague boundaries, a novel deep learning architecture with multi-scale/multi-channel convolution operations (MM-Net) is devised and constructed by a target inversion training method. The MM-Net outperforms other deep learning models in the cell segmentation. Also, a detection/quantification algorithm is developed and implemented to automatically determine the invasiveness of a trapped cell. For the evaluation of the algorithm, it is applied to quantify the invasiveness of breast cancer cells. The results show that the algorithm offers similar performance to the manual calcium analysis method for determining the invasiveness of cancer cells, suggesting that it may serve as a novel tool to automatically determine the invasiveness of cancer cells with high-efficiency. © 2020 Optical Society of America under the terms of the OSA Open Access Publishing Agreement. -
dc.language English -
dc.publisher The Optical Society -
dc.title Fully-automatic deep learning-based analysis for determination of the invasiveness of breast cancer cells in an acoustic trap -
dc.type Article -
dc.identifier.doi 10.1364/BOE.390558 -
dc.identifier.wosid 000561843300007 -
dc.identifier.scopusid 2-s2.0-85085841227 -
dc.type.local Article(Overseas) -
dc.type.rims ART -
dc.description.journalClass 1 -
dc.citation.publicationname Biomedical Optics Express -
dc.contributor.localauthor Youn, Sangyeon -
dc.contributor.localauthor Lee, Kyungsu -
dc.contributor.localauthor Son, Jeehoon -
dc.contributor.localauthor Yang, In-Hwan -
dc.contributor.localauthor Hwang, Jae Youn -
dc.contributor.nonIdAuthor Youn, Sangyeon -
dc.contributor.nonIdAuthor Lee, Kyungsu -
dc.contributor.nonIdAuthor Son, Jeehoon -
dc.contributor.nonIdAuthor Yang, In-Hwan -
dc.identifier.citationVolume 11 -
dc.identifier.citationNumber 6 -
dc.identifier.citationStartPage 2976 -
dc.identifier.citationEndPage 2995 -
dc.identifier.citationTitle Biomedical Optics Express -
dc.type.journalArticle Article -
dc.description.isOpenAccess Y -
dc.subject.keywordPlus TRACKING -
dc.subject.keywordPlus MICROSCOPY -


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