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A new approach for separation of circulating tumor cells (CTCs) in the microfluidics to enhance the capture purity with COMSOL simulation

A new approach for separation of circulating tumor cells (CTCs) in the microfluidics to enhance the capture purity with COMSOL simulation
Alternative Title
마이크로플루이딕을 기반으로 하는 순환 암세포 분리 순도의 증대를 목적으로 하는 COMSOL 시뮬레이션을 이용한 새로운 접근
Lee, Ji Eun
DGIST Authors
Lee, Ji EunMoon, Sang JunPark, Tae Joon
Moon, Sang Jun
Park, Tae Joon
Issued Date
Awarded Date
2015. 2
circulating tumor cells (CTCs)microfluidicsdeformability of cellCOMSOL simulation순환 암세포마이크로플루이딕세포 변형성COMSOL 시뮬레이션
Circulating tumor cells (CTCs) is considered as the indicator of primary cancer of patient since CTCs escape from the primary cancer. The separation and subsequent molecular analysis of CTCs are required to diagnosis, treatment and development of the new drug and biomarker. The microfluidic based separation technology is continuously developed, because of the advantages of microfluidics such as easy handling, low-cost fabrication and integration with optical or electrical systems. The size based hydrodynamic separation technology, especially multi-orifice flow fractionation (MOFF) can offer the high throughout, the high cell viability with labelling process. Also this microfluidic system can isolate CTCs with almost 100% of the capture efficiency, but near to 0% of the capture purity. The problem is the size overlap between a leukocytes and CTCs. The deformability of the cells actually affects to the separation process, however, the deformability of cells is not discussed. To enhance the capture purity, the deformability should be considered, because the cell deformability affects to the result.
In this research, the trajectories of rigid and deformable particles are analyzed using COMSOL simulation. In result, the deformability parameter affects to the change of trajectories of rigid and deformable particles. Moreover the increased fluid velocity and the number of stage enhanced the gap of the trajectories of two types of particles, it should be considered to improve the capture purity as well as the cell size. ⓒ 2015 DGIST
Table Of Contents
1.1 Objectives and Motivations 1--
1.2 Background information 6--
1.3 Current research and principle 25--
1.4 Limitations and Hypothesis 44--
2. Materials and Methods 46--
2.1 Theoretical fundamentals 46--
2.1.1 Fluid flow 46--
2.1.2 Solid mechanics 48--
2.1.3 Fluid solid interaction (FSI) 49--
2.2 Design and simulation 50--
3. Results and Discussion 55--
3.1 Rigid particle and deformable particle in MOFF 55--
3.2 Comparison MOFF and compacted MOFF 60--
3.3 Particle trajectory in compacted MOFF 62--
3.3.1 Focusing position with different fluid velocity 63--
3.3.2 Focusing position with different deformability 65--
3.4 Particle trajectory in ending expansion area 71--
3.4.1 Focusing position with different initial position 72--
3.4.2 Focusing position with different deformability 73--
4. Conclusion 78--
Robotics Engineering
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Department of Robotics and Mechatronics Engineering Theses Master


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