Title

p57 is a molecular switch of adult stem cell quiescence in mouse stomach

DGIST Authors

Somi Kim ; Jong Kyoung Kim ; JaeHyung Koo

Advisor

김종경

Co-Advisor(s)

JaeHyung Koo

Issued Date

2022

Awarded Date

2022/02

Citation

Somi Kim. (2022). p57 is a molecular switch of adult stem cell quiescence in mouse stomach. doi: 10.22677/thesis.200000591934

Type

Thesis

Subject

stomach,gastric chief cells,base stem cells,stem cell quiescence,p57,scRNA-seq

Description

stomach,gastric chief cells,base stem cells,stem cell quiescence,p57,scRNA-seq

Table Of Contents

I. Introduction 1
1.1 The structure of stomach epithelium 1
1.2 The stem cell population in stomach epithelium 1
1.3 Damage response of base stem cells in stomach epithelium 1
II. Preliminary data 2
2.1. Essential gene alterations happen in Troy+ chief cells during injury repair 2
2.2. p57-expressing chief cells directly undergo transition to injury-responsive chief cells upon injury 2
2.3. p57 induces reserve stem cell-like state in gastric corpus organoids 3
III. Methods 3
3.1. Animals 3
3.2. Animal treatments 4
3.3. Library preparation and bulk RNA-seq in Troy+ chief cells 4
3.4. Generation of single-cell RNA-seq data 4
3.5. Time-course RNA-seq data analysis 4
3.6. scRNA-seq data preprocessing 6
3.7. scRNA-seq data analysis 7
IV. Results 8
4.1. Rapid transcriptional changes happen in Troy+ chief cells during injury repair 8
4.2. Single-cell transcriptomic analysis defines a specific injury-responsive chief cell population 11
V. Discussion 16
References 18

URI

http://dgist.dcollection.net/common/orgView/200000591934
http://hdl.handle.net/20.500.11750/16308

DOI

10.22677/thesis.200000591934

Degree

Master

Department

New Biology

Publisher

DGIST

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