p57 is a molecular switch of adult stem cell quiescence in mouse stomach
- Title
- p57 is a molecular switch of adult stem cell quiescence in mouse stomach
- Author(s)
- Somi Kim
- DGIST Authors
- Somi Kim ; Jong Kyoung Kim ; JaeHyung Koo
- Advisor
- 김종경
- Co-Advisor(s)
- JaeHyung Koo
- Issued Date
- 2022
- Awarded Date
- 2022/02
- Type
- Thesis
- Description
- stomach,gastric chief cells,base stem cells,stem cell quiescence,p57,scRNA-seq
- Table Of Contents
-
I. Introduction 1
1.1 The structure of stomach epithelium 1
1.2 The stem cell population in stomach epithelium 1
1.3 Damage response of base stem cells in stomach epithelium 1
II. Preliminary data 2
2.1. Essential gene alterations happen in Troy+ chief cells during injury repair 2
2.2. p57-expressing chief cells directly undergo transition to injury-responsive chief cells upon injury 2
2.3. p57 induces reserve stem cell-like state in gastric corpus organoids 3
III. Methods 3
3.1. Animals 3
3.2. Animal treatments 4
3.3. Library preparation and bulk RNA-seq in Troy+ chief cells 4
3.4. Generation of single-cell RNA-seq data 4
3.5. Time-course RNA-seq data analysis 4
3.6. scRNA-seq data preprocessing 6
3.7. scRNA-seq data analysis 7
IV. Results 8
4.1. Rapid transcriptional changes happen in Troy+ chief cells during injury repair 8
4.2. Single-cell transcriptomic analysis defines a specific injury-responsive chief cell population 11
V. Discussion 16
References 18
- URI
-
http://dgist.dcollection.net/common/orgView/200000591934
http://hdl.handle.net/20.500.11750/16308
- Degree
- Master
- Department
- New Biology
- Publisher
- DGIST
- Related Researcher
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Koo, JaeHyung
- Research Interests 장내미생물/감염균 유래 대사체를 통한 신경염증; 알츠하이머병; 우울증; 당뇨/비만; 대사체/수용체 상호작용에 의한 대사연구
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- Files in This Item:
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- Appears in Collections:
- Department of New Biology Theses Master
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