Cited time in webofscience Cited time in scopus

Brain Endothelial Cells Utilize Glycolysis for the Maintenance of the Transcellular Permeability

Title
Brain Endothelial Cells Utilize Glycolysis for the Maintenance of the Transcellular Permeability
Author(s)
Kim, Eun SeonKim, Kyu-SungLee, Chan HeeJeon, Min-TaeLee, Sung BaeLee, Jong HwaKim, Do-Geun
Issued Date
2022-07
Citation
Molecular Neurobiology, v.59, no.7, pp.4315 - 4333
Type
Article
Author Keywords
Energy metabolismTranscytosisEndothelial cellsGlucoseBBB
Keywords
AEROBIC GLYCOLYSISGLUCOSEMETABOLISMLACTATECANCERTRANSCYTOSISPENETRATIONMECHANISMGLUTAMINENUTRIENT
ISSN
0893-7648
Abstract
Among the components of the blood-brain barrier (BBB), endothelial cells (ECs) play an important role in supplying limited materials, especially glucose, to the brain. However, the mechanism by which glucose is metabolized in brain ECs is still elusive. To address this topic, we assessed the metabolic signature of glucose utilization using live-cell metabolic assays and liquid chromatography-tandem mass spectrometry metabolomic analysis. We found that brain ECs are highly dependent on aerobic glycolysis, generating lactate as its final product with minimal consumption of glucose. Glucose treatment decreased the oxygen consumption rate in a dose-dependent manner, indicating the Crabtree effect. Moreover, when glycolysis was inhibited, brain ECs showed impaired permeability to molecules utilizing transcellular pathway. In addition, we found that the blockade of glycolysis in mouse brain with 2-deoxyglucose administration resulted in decreased transcellular permeability of the BBB. In conclusion, utilizing glycolysis in brain ECs has critical roles in the maintenance and permeability of the BBB. Overall, we could conclude that brain ECs are highly glycolytic, and their energy can be used to maintain the transcellular permeability of the BBB.
URI
http://hdl.handle.net/20.500.11750/16878
DOI
10.1007/s12035-022-02778-7
Publisher
Humana Press, Inc.
Related Researcher
  • 이성배 Lee, Sung Bae
  • Research Interests Cellular mechanism of neurodegenerative diseases; Neuronal maintenance and remodeling; 퇴행성 뇌질환의 세포기전; 신경계 유지 및 리모델링 연구
Files in This Item:

There are no files associated with this item.

Appears in Collections:
Department of Brain Sciences Laboratory of Neurodegenerative Diseases and Aging 1. Journal Articles

qrcode

  • twitter
  • facebook
  • mendeley

Items in Repository are protected by copyright, with all rights reserved, unless otherwise indicated.

BROWSE