Full metadata record
DC Field | Value | Language |
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dc.contributor.author | Kim, Hye Young | - |
dc.contributor.author | Lee, Sung Bae | - |
dc.contributor.author | Kang, Hyen Sam | - |
dc.contributor.author | Oh, Goo Taeg | - |
dc.contributor.author | Kim, TaeSoo | - |
dc.date.available | 2017-07-05T08:53:08Z | - |
dc.date.created | 2017-04-10 | - |
dc.date.issued | 2014-06 | - |
dc.identifier.issn | 0006-291X | - |
dc.identifier.uri | http://hdl.handle.net/20.500.11750/2388 | - |
dc.description.abstract | Flo8 is a transcriptional activator essential for the inducible expression of a set of target genes such as STA1, FLO11, and FLO1 encoding an extracellular glucoamylase and two cell surface proteins, respectively. However, the molecular mechanism of Flo8-mediated transcriptional activation remains largely elusive. By generating serial deletion constructs, we revealed here that a novel transcriptional activation domain on its extreme C-terminal region plays a crucial role in activating transcription. On the other hand, the N-terminal LisH motif of Flo8 appears to be required for its physical interaction with another transcriptional activator, Mss11, for their cooperative transcriptional regulation of the shared targets. Additionally, GST pull-down experiments uncovered that Flo8 and Mss11 can directly form either a heterodimer or a homodimer capable of binding to DNA, and we also showed that this formed complex of two activators interacts functionally and physically with the Swi/Snf complex. Collectively, our findings provide valuable clues for understanding the molecular mechanism of Flo8-mediated transcriptional control of multiple targets. © 2014 Elsevier Inc. All rights reserved. | - |
dc.publisher | Academic Press Inc. | - |
dc.title | Two distinct domains of Flo8 activator mediates its role in transcriptional activation and the physical interaction with Mss11 | - |
dc.type | Article | - |
dc.identifier.doi | 10.1016/j.bbrc.2014.04.161 | - |
dc.identifier.scopusid | 2-s2.0-84902078118 | - |
dc.identifier.bibliographicCitation | Biochemical and Biophysical Research Communications, v.449, no.2, pp.202 - 207 | - |
dc.subject.keywordAuthor | Flo8 | - |
dc.subject.keywordAuthor | Transcriptional activation | - |
dc.subject.keywordAuthor | Mss11 | - |
dc.subject.keywordAuthor | FLO1 | - |
dc.subject.keywordAuthor | FLO11 | - |
dc.subject.keywordAuthor | LisH motif | - |
dc.subject.keywordPlus | SACCHAROMYCES-CEREVISIAE | - |
dc.subject.keywordPlus | PSEUDOHYPHAL DIFFERENTIATION | - |
dc.subject.keywordPlus | GLUCOSE REPRESSION | - |
dc.subject.keywordPlus | NUCLEAR-PROTEIN | - |
dc.subject.keywordPlus | GENE-EXPRESSION | - |
dc.subject.keywordPlus | INVASIVE GROWTH | - |
dc.subject.keywordPlus | COMPLEX | - |
dc.subject.keywordPlus | KINASE | - |
dc.subject.keywordPlus | RECRUITMENT | - |
dc.subject.keywordPlus | PROMOTER | - |
dc.citation.endPage | 207 | - |
dc.citation.number | 2 | - |
dc.citation.startPage | 202 | - |
dc.citation.title | Biochemical and Biophysical Research Communications | - |
dc.citation.volume | 449 | - |
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